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Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml/接骨木凝集素,琼脂糖交联
品牌: Vectorlabs
产品货号: AL-1303-2
规格: 2 ml

报价:
折后价:

数量: - +
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml/接骨木凝集素,琼脂糖交联
  • 别名:
    接骨木凝集素,琼脂糖交联
  • 产品描述:
    Sambucus nigra lectin SNA, EBL, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.Agarose bound* Sambucus nigra lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.This coupling method provides several advantages over the traditional cyanogen bromide procedure:Maximum carbohydrate binding activity of the coupled lectins is retainedLinkage is stable over a range of pH valuesConjugated proteins are not leached off the beads by Tris or other routinely used buffersNo residual charges are present after conjugation. This minimizes non-specific binding to the matrix.Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid*3 mg lectin/ml gel
  • 反应种属:
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  • 实验应用:
    Glycobiology,Affinity Chromatography
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  • 推荐稀释度:
    Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-7100. Alternatively, 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage.
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  • 偶联:
    Agarose
  • 产品浓度:
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  • 保存温度:
    2° - 8°C – Do Not Freeze
  • 运输温度:
    Ambient
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  • 存储溶液:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 20 mM lactose
  • 有效期:
  • 产地:
    美国
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  • 样本类型:
    10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 20 mM lactose
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