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Streptavidin, Fluorescein, 1 mg/链霉亲和素,FLUORESCEIN标记
品牌: Vectorlabs
产品货号: SA-5001-1
规格: 1 mg

报价:
折后价:

数量: - +
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    Streptavidin, Fluorescein, 1 mg/链霉亲和素,FLUORESCEIN标记
  • 别名:
    链霉亲和素,FLUORESCEIN标记
  • 产品描述:
    Fluorescein Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.Streptavidin, fluorescein labeled, has an excitation maximum at 495 nm and an emission maximum at about 515 nm. This product has an optimal fluorochrome to protein ratio and is one of the reagents we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent techniques, Fluorescein Streptavidin can provide greater sensitivity and lower background staining.
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  • 实验应用:
    Immunofluorescence,In situ hybridization,Flow Cytometry/Cell Separation
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  • 推荐稀释度:
    For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30µg/ml.
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  • 偶联:
    Fluorescein
  • 产品浓度:
    1.0 mg active conjugate/ml
  • 表达系统:
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  • 保存温度:
    2° - 8°C – Do Not Freeze
  • 运输温度:
    Ambient
  • 细胞定位:
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  • 产品形式:
    Concentrate
  • 存储溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
  • 有效期:
  • 产地:
    美国
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  • 样本类型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
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