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BrdU系列产品精选!

2021-05-18
浏览次数: 8
关于BrdU
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU),中文名为5-溴脱氧尿嘧啶核苷,是一种合成的核苷,胸腺嘧啶(thymidine)的类似物。BrdU普遍使用于检测活组织中的增殖细胞,对研究细胞动力学有重要意义。
BrdU可被正在复制的细胞(S期)所摄取,整合至新合成的DNA链中,可取代胸腺嘧啶(thymidine)。然后可用BrdU特异性的抗体来检测被摄入的BrdU,以此来反应细胞的增殖。DNA须经过变性(denaturation)才可与抗体结合,通常会用酸或热来处理细胞。
 
 
工欲善其事,必先利其器,BrdU检测工具,您了解多少?
 
BrdU细胞增殖ELISA检测试剂盒
Exalpha公司研发的Frontier™ BrdU 细胞增殖检测分析试剂盒,是一种完全适用于高通量应用、非同位素标记法的ELISA检测试剂盒(可提供显色法和化学发光法),可检测掺入到贴壁细胞及非贴壁细胞新合成DNA链中的BrdU。
 
试剂盒检测原理
主要以细胞层作为固相载体, 将BrdU摄入微孔板中培养的细胞。在细胞培养的最后2-14小时,把BrdU加入微孔板中,BrdU将被掺入到分裂细胞的DNA中。为确保抗体与BrdU有效结合,需要将细胞固定并做破膜处理,对DNA进行变性。所有操作仅需通过一步固定剂(Fixing solution)处理即可。将BrdUd单克隆抗体加入到板孔中,孵育1小时,未结合的抗体通过洗涤步骤去除,然后加入HRP标记的羊抗小鼠二抗,最后加入HRP底物显色。
 
 名称 货号 规格 品牌
 Frontier™ Bromodeoxyuridine (BrdU)
 Cell Proliferation ELISA Kit(比色法)
 X1327K1  200 tests Exalpha
 Frontier™ Bromodeoxyuridine (BrdU)
 Cell Proliferation ELISA Kit(比色法)
 X1327K2  1000 tests Exalpha
 Frontier™ Bromodeoxyuridine (BrdU)
 Cell Proliferation ELISA Kit(比色法)
 X1327K3 5000 tests Exalpha
 Frontier™ Chemiluminescent
 Bromodeoxyuridine (BrdU) Cell
 Proliferation ELISA Kit(化学发光法)
 X1623K1 200 tests Exalpha
 Frontier™ Chemiluminescent
 Bromodeoxyuridine (BrdU) Cell
 Proliferation ELISA Kit(化学发光法)
 X1623K2 1000 tests Exalpha
 Frontier™ Chemiluminescent
 Bromodeoxyuridine (BrdU) Cell
 Proliferation ELISA Kit(化学发光法)
 X1623K3 5000 tests Exalpha
 
BrdU免疫组化检测试剂盒
BrdU被掺入到分裂细胞的DNA中,然后加生物素化的BrdU特异性抗体孵育,再通过HRP-链亲和素结合物催化DAB底物显色。该试剂盒可用对任何种属的组织进行染色。
 
 名称 货号 规格 品牌
 Frontier™ Bromodeoxyuridine (BrdU) Immunohistochemistry Kit X1545K 50 slides Exalpha
 
BrdU流式染色试剂盒
 名称 应用 货号 品牌
 BrdU Staining Buffer Set FC 00-5525 eBioscience
 BrdU Staining Kit for Flow Cytometry APC FC 8817-6600 eBioscience
 BrdU Staining Kit for Flow Cytometry eFluor® 450 FC 8848-6600 eBioscience
 BrdU Staining Kit for Flow Cytometry FITC FC 8811-6600 eBioscience
 BrdU Staining Kit for Flow Cytometry PE FC 8812-6600 eBioscience
 BrdU Staining Kit for Flow Cytometry PerCP-eFluor® 710 FC 8846-6600 eBioscience

BrdU抗体
名称货号规格品牌
Sheep anti BrdU Polyclonal AntibodyA205P 250 µgExalpha
Mouse Anti-Bromodeoxyuridine Monoclonal AntibodyX1028100 µgExalpha
Mouse Anti-Bromodeoxyuridine Monoclonal Antibody(FITC conjugate)X1543M100 µgExalpha
RAT ANTI BrdUMCA20600.2 mgAbD Serotec
RAT ANTI BrdU:Alexa Fluor® 488MCA2060A488100testsAbD Serotec
RAT ANTI BrdU:BiotinMCA2060B0.1 mgAbD Serotec
RAT ANTI BrdU:FITCMCA2060F0.1 mgAbD Serotec
RAT ANTI BrdU:FITCMCA2060FT0.5 mlAbD Serotec
RAT ANTI BrdUMCA2060GA0.1 mgAbD Serotec
RAT ANTI BrdU:HRPMCA2060P0.5 mgAbD Serotec
RAT ANTI BrdU:HRPMCA2060PA0.1 mgAbD Serotec
RAT ANTI BrdUMCA2060T20 µgAbD Serotec
MOUSE ANTI BrdUMCA24830.2 mgAbD Serotec
MOUSE ANTI BrdU:FITCMCA2483FA50 µgAbD Serotec
MOUSE ANTI BrdUMCA2483T20 µgAbD Serotec
RAT ANTI BrdUOBT00300.5 mgAbD Serotec
RAT ANTI BrdUOBT0030CX0.25 mlAbD Serotec
RAT ANTI BrdU:FITCOBT0030F1 mlAbD Serotec
RAT ANTI BrdUOBT0030G0.25 mgAbD Serotec
RAT ANTI BrdUOBT0030S5 mlAbD Serotec
BrdU [MoBu-1] antibody (Biotin)GTX10362100μgGeneTex
BrdU antibody [BU6-4]GTX1221950μgGeneTex
BrdU antibodyGTX128091100μlGeneTex
BrdU antibody [BU1/75 (ICR1)]GTX26326100μgGeneTex
BrdU antibody [BU1/75 (ICR1)] (FITC)GTX27384250μlGeneTex
BrdU antibody [B33.1]GTX27781500μlGeneTex
BrdU antibody [B684M]GTX44314100μgGeneTex
BrdU antibody [IIB5] (FITC)GTX50089500μlGeneTex
BrdU, mAb (2B1)ADI-MSA-200-E1*100ugENZO
5-Bromodeoxyuridine, mAb (PRB-1) (biotin conjugate) ALX-804-197B-T06060 tests ENZO
5-Bromodeoxyuridine, mAb (PRB-1) (fluorescein conjugate) ALX-804-197L-T06060 tests ENZO
Anti-BrdU APC17-507125/100testseBioscience
Anti-BrdU eFluor® 45048-507125/100testseBioscience
Anti-BrdU FITC11-507125/100testseBioscience
Anti-BrdU PE12-507125/100testseBioscience
Anti-BrdU PerCP-eFluor® 71046-507125/100testseBioscience
Anti-BrdU Purified14-507125/100ugeBioscience





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