深圳欣博盛生物科技有限公司

S100P Antibody / Protein S100-P

S100P antibody detects Protein S100-P (also known as S100 calcium-binding protein P), a member of the S100 family of calcium-binding proteins that regulate diverse cellular processes including cell growth, differentiation, cytoskeletal dynamics, and signal transduction. Encoded by the S100P gene on chromosome 4p16, this small acidic protein contains two EF-hand calcium-binding motifs that undergo conformational change upon calcium binding, enabling interaction with target proteins. Through these calcium-dependent interactions, S100P influences intracellular signaling cascades and modulates cytoskeletal organization, motility, and transcriptional activity.

S100P is expressed in multiple tissues, including placenta, pancreas, lung, and prostate, and is frequently upregulated in cancers where it contributes to tumor progression and metastasis. The protein interacts with the receptor for advanced glycation end products (RAGE) at the cell surface, activating downstream MAPK and NF-kB pathways that promote proliferation, migration, and resistance to apoptosis. Elevated S100P expression has been associated with poor prognosis in breast, pancreatic, and colorectal carcinomas, making it a biomarker for aggressive tumor phenotypes and potential therapeutic target.

The S100P antibody is widely used in cancer biology and molecular pathology to study calcium signaling and metastasis regulation. In western blot analysis, S100P appears as an approximately 11 kilodalton band corresponding to the monomeric form, while immunohistochemistry reveals both cytoplasmic and nuclear localization depending on cell type. In cancer diagnostics, S100P immunoreactivity is employed to distinguish pancreatic ductal adenocarcinoma from benign pancreatic lesions and to evaluate metastatic spread. The antibody is also valuable in studies of placental function and epithelial differentiation where S100P participates in hormone-responsive signaling networks.

Beyond oncology, S100P contributes to inflammation and tissue repair. It enhances fibroblast motility and collagen remodeling through calcium-dependent activation of small GTPases such as Rac1 and Cdc42. It also regulates gene transcription by interacting with nuclear receptors and coactivators, influencing expression of stress-response and survival genes. The S100P antibody enables detailed characterization of these pathways, providing insight into the molecular mechanisms that couple calcium signaling to cell behavior. Researchers employ it in western blotting, immunofluorescence, and immunohistochemistry to quantify expression and localization in tissue or cultured cells.

Because of its strong link to metastasis, S100P has emerged as a therapeutic target and diagnostic biomarker. Inhibitors of the S100P-RAGE interaction are under investigation for their potential to reduce tumor invasion and inflammation. The S100P antibody supplied by NSJ Bioreagents is validated for high specificity and reproducibility, supporting applications in cancer research, calcium signaling studies, and biomarker development. Its ability to detect S100P in both human and model systems makes it a versatile reagent for translational research aimed at understanding calcium-regulated cellular processes.

Human

Rabbit

WB, ICC, IF, FACS

Rabbit IgG

A synthetic peptide corresponding to a sequence at the C-terminus of human S100P was used as the immunogen for the S100P antibody.

Polyclonal (rabbit origin)

Immunogen affinity purified

After reconstitution, the S100P antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

美国