天然免疫学热点领域科研抗体(AbD Serotec®)---巨噬细胞标志物抗体
AbD Serotec公司是全球领先的巨噬细胞标志物抗体供应商，其生产的MCA497, Rat Anti Mouse F4/80 Clone CI:A3-1是著名的成熟小鼠巨噬细胞表面特异性标志物抗体，有将近400篇的文献引用，提供多达30种的包装形式，可用于FC、WB，IF、IHC等7种应用。AbD Serotec享有该克隆的专利，是该抗体唯一的商业化生产商。
MCA1957识别小鼠细胞表面macrosialin，为人CD68的同源物，是专门表达于组织巨噬细胞的一种重度糖基化的跨膜糖蛋白。
小鼠淋巴结的免疫荧光染色；巨噬细胞用anti 小鼠脾脏冰冻切片的免疫组化染色；红髓中的
F4/80(MCA497PE)染成红色，B细胞用anti C- 巨噬细胞用anti CD68(MCA1957)染色
D79b(MCA1821A488)染成绿色，细胞核用D-
API复染，呈蓝色
AbD Serotec公司提供的最受欢迎的巨噬细胞标志物抗体
人巨噬细胞标志物抗体 | |||
产品名称 | 克隆号 | 产品编号 | 形式 |
CD11b | ICRF44 | MCA551 | Pur., A647, A700, Az. Fr., Biot., FITC, RPE, RPE-A647 |
CD14 | UCHM1 | MCA596 | Pur., A647, APC, Az. Fr., Biot., FITC, RPE |
CD14 | TUK4 | MCA1568 | Pur., A647, A700, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750 |
CD14 | MEM-18 | MCA2185 | Pur., FITC, RPE |
CD14 | 61D3 | MCA2804 | RPE-Cy5, RPE-Cy5.5 |
CD163 | EDHu-1 | MCA1853 | Pur., FITC |
CD169 | 7-239 | MCA2517 | Pur., A488, A647, FITC, LE, RPE |
CD200R | OX108 | MCA2282 | Pur., A488, A647, Biot., FITC, RPE |
CD204 | Poly | AHP563 | Serum |
CD206 | 15-2 | MCA2155 | Pur. |
CD209 | MR-1 | MCA2318 | Pur., A488, A647, Biot., FITC, LE, RPE |
CD31 | WM59 | MCA1738 | Pur., A647, Biot., FITC, RPE |
CD36 | SMØ | MCA722 | Pur., A647, Az. Fr., FITC |
CD68 | 514H12 | MCA1815 | S/N |
CD68 | KiM7 | MCA2375F | FITC |
Dectin-1 | BD6 | MCA4662 | Pur., A488, A647, FITC, RPE |
Dectin-1 | GE2 | MCA4661 | Pur., A488, A647, FITC, LE, RPE |
Macrophage | MAC387 | MCA874 | Pur., A488, FITC, LE, RPE |
小鼠巨噬细胞标志物抗体 | |||
CD11b | M1/70.15 | MCA74 | Pur., A488, A647, A700, Biot., FITC, LE, RPE, RPE- |
CD11b | 5C6 | MCA711 | Pur., FITC, LE, RPE |
CD115 | 604B5 | 2E11 | MCA1898 Pur., Az. Fr., RPE |
CD169 | MOMA-1 | MCA947 | Pur., S/N, A647, FITC |
CD169 | 3D6.112 | MCA884 | Pur., FITC, LE |
CD200R | OX-110 | MCA2281 | Pur., A488, A647, Biot., FITC, LE, RPE |
CD204 | 2F8 | MCA1322 | Pur., A488, A647, Biot., FITC, LE, RPE |
CD206 | MR5D3 | MCA2235 | Pur., A488, A647, Biot., FITC, LE, RPE |
CD31 | ER-MP12 | MCA2388 | Pur., A488, A647, Biot., RPE, RPE-A647 |
CD36 | MF3 | MCA2748 | Pur., A488, A647, FITC, LE, RPE |
CD68 | FA-11 | MCA1957 | Pur., A488, A647, A700, Az. Fr., Biot., FITC, RPE |
Dectin-1 | 2A11 | MCA2289 | Pur., A647, Biot., FITC, LE, RPE |
Dectin-2 | D2.11E4 | MCA2415 | Pur., A488, A647, Biot., FITC, LE, RPE |
F4/80 | CI:A3-1 | MCA497 | Pur., A488, A647, A700, APC, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750, S/N |
Ly-6B.2 | 7/4 | MCA771 | Pur., A647, A700, Biot., FITC, LE, RPE |
Ly-6C | ER-MP20 | MCA2389 | Pur., A488, A647, Biot., RPE |
MARCO | ED31 | MCA1849 | Pur., FITC, RPE |
TREM-1 | L5-B8.2A12.3A12 | MCA2578 | Pur., A488, A647, FITC, LE, RPE |
TREM-2 | 78.18 | MCA4772 | Pur., FITC, LE |
大鼠巨噬细胞标志物抗体 | |||
CD11b | OX-42 | MCA275 | Pur., A488, A647, A700, Biot., FITC, LE, PB, RPE, RPE- |
CD11b | ED7 | MCA618R | Pur. |
CD11b | ED8 | MCA619 | Pur., FITC, RPE |
CD115 | 604B52E11 | MCA1898 | Pur., Az. Fr., RPE |
CD163 | ED2 | MCA342 | Pur., A647, Biot., FITC, LE, RPE |
CD169 | ED3 | MCA343 | Pur. |
CD172a | OX41 | MCA274 | Pur., Az. Fr., Biot, FITC, RPE |
CD172a | ED9 | MCA620 | Pur., FITC, RPE |
CD200R1 | OX-102 | MCA1959 | Pur., Biot., FITC, LE, RPE |
CD31 | TLD-3A12 | MCA1334 | Pur., A488, A647, Biot., FITC, LE, RPE |
CD68 | ED1 | MCA341 | Pur., A488, A647, A700, Biot., FITC, RPE |
荧光多色染色试剂
产品名称 | 克隆号 | 产品编号 | 形式 |
Human CD45/CD14 | F10-89-4/UCHM1 | DC008 | FITC/RPE |
Mouse IgG2a/IgG2a | Negative Control | DC013 | FITC/RPE |
Mouse Ly6C/CD31 | ERMP20/ERMP12 | DC053 | A488/RPE |
Mouse Ly6C/CD31 | ERMP20/ERMP12 | DC054 | A488/RPE-A647 |
Mouse CD11b/CD115 | M1/70.15 / 604B5 2E11 | DC055 | A488/RPE |
Mouse Ly6C/CD115/CD11b | ERMP20/604B52E11/M1/70.15 | TC015 | A488/RPE/RPE-A647 |
Mouse Ly6C/CD115/CD31 | ERMP20/604B52E11/ERMP12 | TC016 | A488/RPE/RPE-A647 |
Mouse Ly6C/CD115/CD31 | ERMP20/604B52E11/ERMP12 | TC017 | A488/RPE/A647 |
Mouse Ly6C/CD115/CD11b/CD31 | ERMP20/604B52E11/M1/70.15/ERMP12 | QC001 | A488/RPE/RPE-A647/A647 |
AbD Serotec公司 F4/80 产品参考文献
1. Austyn, J.M. and Gordon, S. (1981) F4/80 , a monoclonal antibody directed specifically against the mouse macrophage.Eur. J. Immunol. 11: 805-815.
2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia.The Anatomical Record 210: 503-512.
3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.J. Exp. Med. 161: 475-489.
4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr. Top. Micro. Immunol. 181: 1-37.
5. Uaesoontrachoon, K. et al. (2013) Osteopontin deficiency delays inflammatory infiltration and the onset of muscle regeneration in a mouse model of muscle injury.Dis Model Mech. 6: 197-205.
6. Lin, H. H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.J. Exp. Med. 201: 1615–1625.
7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor.Blood. 105: 3737-3742.
8. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.J. Physiol. 562:899-913.
9. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.J Biol Chem. 286: 19641-51.
10. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
J Exp Med. 209: 139-55.
11. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member,Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linkedmarker for subsets of mouse macrophages and dendritic cells.J Immunol. 182:1278-86.
12. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study.Exp Diabetes Res. 2010: 476969.
13. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.Nat Immunol. 11: 1136-42.
14. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance.Endocrinology. 152: 836-46.
15. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system ininnate brain inflammation.Glia. 57: 1802-14.
16. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.Am J Physiol Endocrinol Metab. 303: E597-606.
17. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS One. 7: e42654.
18. Banda, N.K. et al. (1012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.J Immunol. 188: 1469-78.
19. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.BMC Cancer. 12: 35.
20. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.Gastroenterology. 138: 2399-409,
21. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.PLoS One. 8: e68893.
22. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1a in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.PLoS One. 9(4):e93856.
