深圳欣博盛生物科技有限公司

Carf Antibody / Collaborator of ARF / Cdkn2aip

Carf antibody detects Collaborator of ARF, a nuclear protein encoded by the CDKN2AIP gene on chromosome 4q35.1. CARF (CDKN2A-interacting protein) acts as a transcriptional co-regulator and cell cycle checkpoint factor that mediates the tumor suppressor function of p14ARF and p53. It plays key roles in cellular senescence, DNA damage response, and cell proliferation control. CARF is expressed in most proliferative tissues, including liver, kidney, and hematopoietic cells, with particularly high levels in dividing and stress-activated cells.

Structurally, CARF contains a coiled-coil domain and nuclear localization signals that facilitate interaction with chromatin-associated proteins and transcriptional regulators. It belongs to the CDKN2A-interacting protein family and acts as a bridge between the ARF tumor suppressor pathway and p53 signaling. CARF directly binds p14ARF and modulates p53 stability through MDM2 regulation, coordinating cell cycle arrest in response to genotoxic stress. Co-localization studies show CARF within the nucleus and nucleolus, where it associates with replication machinery and chromatin-modifying complexes.

Functionally, CARF serves as a cell cycle regulator that maintains p53 activity and prevents uncontrolled proliferation. Under DNA damage conditions, CARF enhances p53-dependent transcription of target genes such as CDKN1A (p21), promoting cell cycle arrest and senescence. It also regulates replication stress responses by interacting with checkpoint proteins such as ATR and CHK1. CARF expression levels determine whether a cell undergoes reversible arrest or permanent senescence, integrating multiple stress and growth signals.

Dysregulation of CARF expression has been implicated in cancer and aging. Overexpression can suppress proliferation and induce senescence, while loss of CARF may impair p53 function and enhance oncogenic transformation. In certain tumors, CARF mislocalization correlates with altered cell cycle control. Pathway associations include p53 signaling, DNA damage response, and cell cycle checkpoint regulation. Developmentally, CARF contributes to tissue homeostasis by regulating stem and progenitor cell proliferation.

The Carf antibody from NSJ Bioreagents is a valuable reagent for studies of tumor suppression, senescence, and p53 pathway regulation.

Mouse, Rat

Rabbit

WB, FACS, ELISA

Rabbit IgG

E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689) was used as the immunogen for the Carf antibody.

Polyclonal (rabbit origin)

Immunogen affinity purified

After reconstitution, the Carf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

美国