EP2 Receptor (rat) Reporter Assay Kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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EP2 Receptor (rat) Reporter Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：600340

规格：100 tests

目录价：￥6847.4

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

EP2 Receptor (rat) Reporter Assay Kit

别名:

PGE2 Receptor 2|Prostaglandin E2 Receptor 2

产品描述:

The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP2 Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP2 receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP2 Receptor Reverse Transfection Plate). Cells grown on the transfection complex will express EP2 at the cell surface. Binding of agonists to EP2 initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP2. PGE2 is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.

保存温度:

-20

运输温度:

-20

有效期:

365

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