izTRAIL, Soluble (human) (rec.)-其他重组蛋白-深圳欣博盛生物科技有限公司

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izTRAIL, Soluble (human) (rec.)

品牌：Adipogen

官网链接

说明书

折扣/货期

货号：AG-40B-0069-5010	规格：5 x 10 ug	目录价：￥6480
货号：AG-40B-0069-C010	规格：10 ug	目录价：￥2160

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

izTRAIL, Soluble (human) (rec.)

别名:

Apo-2L; TNFSF10; CD253

产品描述:

izTRAIL is a newly available, highly active recombinant form of soluble human TRAIL. Due to a trimerizing N-terminal isoleucine zipper (iz) motif the intrinsic trimerization of TRAIL, required for apoptosis-inducing activity of TRAIL, is enhanced when compared to non-tagged soluble human TRAIL (shTRAIL). Therefore, izTRAIL is a potent inducer of apoptosis in many human cancer cells, but not normal human hepatocytes. In addition, the half-life of izTRAIL is about eight-fold higher than the half-life of shTRAIL. These properties render izTRAIL highly suitable for both, in vitro and in vivo use, particularly for studies in which investigators plan to transfer their in vitro results into an in vivo system with human cancer cells in xenotransplant settings examining susceptibility to TRAIL-induced apoptosis.

宿主来源:

E. coli

靶标/特异性:

Binds to human TRAIL receptors 1-4 (TRAIL-R1 to TRAIL-R4); izTRAIL does not interact with the apoptosis-inducing mouse TRAIL receptor (TRAIL-R).

产品浓度:

0.1mg/ml after reconstitution

保存温度:

+4°C

运输温度:

预期分子量:

~82kDa as stable trimers (determined by size exclusion chromatography) ~28kDa as monomer (determined by SDS-PAGE)

产品形式:

solid

存储溶液:

Lyophilized. Contains 20mM TRIS-Cl, 0.5M arginine-HCl, 100mM NaCl, 0.02% Tween 20.

产地:

瑞士

纯度:

>95% (SDS-PAGE)

生物活性:

Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1µg/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500µg per day (Wissink 2006).

内毒素水平:

<0.01EU/µg purified protein (LAL test; Lonza).

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