深圳欣博盛生物科技有限公司

JADE1 Antibody / Jade family PHD finger protein 1 / PHF17

JADE1 antibody detects Jade family PHD finger protein 1, a chromatin-associated transcriptional regulator encoded by the JADE1 gene on chromosome 4q28.2. JADE1 belongs to the JADE protein family and plays critical roles in histone acetylation, gene expression, and renal epithelial cell differentiation. It functions as a component of the HBO1 (histone acetyltransferase binding to ORC1) complex, which acetylates histones H4 and H2A to promote chromatin remodeling and transcriptional activation. JADE1 is expressed in kidney, liver, and epithelial tissues, with strong enrichment in renal tubular cells where it supports differentiation and polarity maintenance.

Structurally, JADE1 contains two PHD (plant homeodomain) zinc finger motifs, an N-terminal coiled-coil domain, and a C-terminal PEST sequence important for protein stability. These domains facilitate chromatin binding and complex formation with HBO1, ING4, and BRPF family proteins. JADE1 belongs to the chromatin-modifying family of histone acetylation regulators that modulate epigenetic transcriptional control. Co-localization studies show JADE1 primarily localized to the nucleus, especially at transcriptionally active chromatin regions.

Functionally, JADE1 promotes histone acetylation and chromatin accessibility, facilitating the expression of genes involved in cell cycle regulation, epithelial morphogenesis, and DNA repair. It stabilizes the HBO1 complex and coordinates acetyltransferase activity at replication origins and promoters. In kidney epithelial cells, JADE1 regulates Wnt and TGF-beta pathways to maintain differentiation and suppress epithelial-to-mesenchymal transition (EMT). JADE1 also interacts with von Hippel-Lindau (VHL) tumor suppressor protein, enhancing the degradation of oncoproteins such as beta-catenin and cyclin E, linking it to tumor suppression mechanisms.

Loss or mutation of JADE1 disrupts chromatin organization and promotes tumorigenesis, particularly in renal cell carcinoma and hepatocellular carcinoma. Reduced JADE1 levels correlate with increased proliferation and dedifferentiation of epithelial cells. Pathway associations include histone acetylation, transcriptional regulation, and Wnt signaling. During development, JADE1 expression supports epithelial lineage specification and organogenesis. Known binding partners include HBO1, ING4, BRPF3, and VHL, forming multi-protein chromatin remodeling assemblies.

The JADE1 antibody from NSJ Bioreagents is an excellent reagent for studying chromatin regulation, epithelial differentiation, and tumor suppression mechanisms.

Human

Rabbit

WB, ICC, IF, FACS, ELISA

Rabbit IgG

E.coli-derived human PHF17/JADE1 recombinant protein (Position: I93-D811) was used as the immunogen for the JADE1 antibody.

Polyclonal (rabbit origin)

Immunogen affinity purified

After reconstitution, the JADE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

美国