MitoCheck® Mitochondria (Cell Culture) Isolation Kit-其他特色试剂盒-深圳欣博盛生物科技有限公司

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MitoCheck® Mitochondria (Cell Culture) Isolation Kit

品牌：Cayman

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折扣/货期

货号：502871

规格：1 ea

目录价：￥4355

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产品名称:

MitoCheck® Mitochondria (Cell Culture) Isolation Kit

产品描述:

Cayman’s MitoCheck® Mitochondria (Cell Culture) Isolation Kit enables the isolation of functionally intact mitochondria from cultured cells in less than 60 minutes. Cells are initially resuspended in an isotonic buffer and subjected to mechanical disruption to liberate organelles. This is followed by sequential differential centrifugation to first remove nuclei and undisrupted cells and subsequently pellet mitochondria.An optional digitonin cell permeabilization reagent is also provided to facilitate plasma membrane disruption, which may enable increased mitochondrial yields. Digitonin mediates selective disruption of cholesterol-rich membranes, resulting in plasma membrane permeabilization, while mitochondrial membranes, which are comparatively low in cholesterol, remain intact.{71328}This kit provides sufficient reagents to perform up to 60 isolations. Mitochondria isolated using this kit are functionally intact and may be used immediately for respirometry studies or other downstream application. Additional Cayman MitoCheck® assay kits may be used to characterize mitochondrial function.

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来源:

Animal/Bovine

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