| 货号:702450 | 规格:96 wells | 目录价:¥6633 |
产品详情
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产品名称:
NADPH/NADP Ratio Assay Kit
产品描述:
Cayman’s NADPH/NADP Ratio Assay Kit provides a colorimetric method for measuring NADP+, NADPH, and their ratio in cell culture extracts. In this assay, NADP+ is enzymatically converted to NADPH by glucose-6-phosphate dehydrogenase (G6PDH), which leads to the reduction of the tetrazolium salt substrate WST-8 to a highly colored formazan that absorbs at 450 nm. The amount of formazan produced is proportional to the amount of NADP+ and NADPH in the matrix. To measure NADPH specifically, samples are heated to degrade NADP+. The NADP+ concentration is then calculated by subtracting the signal obtained from the heated sample from that of the corresponding non-heated sample. This assay has a range of 0-1.5 µM and a lower limit of detection (LLOD) of 0.02 µM.
保存温度:
-20
运输温度:
-20
有效期:
365
检测范围:
0-1.5 µM
来源:
Animal/Bovine|Microorgansim
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