ELISpot Plus: Human IFN-γ (ALP)
名称 | ELISpot Plus: Human IFN-γ (ALP) |
货号 | 3420-4APT-2、3420-4APT-10、3420-4APT-100 |
规格 | 2 plates、10 plates、100 plates |
应用 | ELISpot |
保存温度 | 抗体、酶结合物和底物建议在4-8 °C下保存;平板可在室温下保存 |
试剂盒组分 | 预包被板 检测抗体:7-B6-1, biotin (40 ul, 1mg/ml) 酶结合物:Streptavidin-ALP (40 ul) 阳性对照:Anti-CD3 mAb (CD3-2) (100 ul) 底物:BCIP/NBT-plus substrate (25 ml) |
说明书 | https://www.mabtech.com/products/elispot-plus-human-ifn-g-alp-3420-4ast-4-0 |
示例1:
在有或无抗-CD3 单克隆抗体(货号:3605-1-50)的情况下,将PBMC(62500 个细胞/孔)培养20小时,然后用ELISpot分析分泌IFN-γ的细胞数量。
示例2:
在无或有CEF多肽池(货号:3616-1)的情况下,将PBMC(250000个细胞/孔)培养20小时,然后用ELISpot 分析分泌IFN-γ的细胞数量。
ELISpot Plus与ELISpot Pro的区别
ELISpot Plus | ELISpot Pro | |
ELISpot plate | Pre-coated | Pre-coated |
Capture mAb | Coated on plate | Coated on plate |
Detection mAb ALP/HRP | - | √ |
Detection mAb, biotinylated | √ | - |
Streptavin-ALP/HRP | √ | - |
Substrate | √ | √ |
Anti-CD3 mAb (positive control) | √ | √ |
Size | 2, 10, and 100 plates | 2, 10, and 100 plates |
部分引用文献:
1. Yang, Jingyun et al. “Low levels of neutralizing antibodies against XBB Omicron subvariants after BA.5 infection.” Signal transduction and targeted therapy vol. 8,1 252. 19 Jun. 2023, doi:10.1038/s41392-023-01495-4
2. Shroff, Rachna T et al. “Immune Responses to COVID-19 mRNA Vaccines in Patients with Solid Tumors on Active, Immunosuppressive Cancer Therapy.” medRxiv : the preprint server for health sciences 2021.05.13.21257129. 25 Aug. 2021, doi:10.1101/2021.05.13.21257129. Preprint.
3. Melgaço, Fabiana Gil et al. “Impairment of CD4+ T and Memory B Cell Responses but Normal Memory CD8+T-Cell Activation on Crohn's Disease after COVID-19 Vaccination: A Twin Case.” Viruses vol. 13,11 2143. 24 Oct. 2021, doi:10.3390/v13112143
4. Zhou, Runhong et al. “Acute SARS-CoV-2 Infection Impairs Dendritic Cell and T Cell Responses.” Immunity vol. 53,4 (2020): 864-877.e5. doi:10.1016/j.immuni.2020.07.026
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✦ 品牌简介
Mabtech是一家瑞典生物科技公司,1986年起源于斯德哥尔摩大学免疫学系,是ELISpot和T细胞检测技术的全球领先者。Mabtech公司以前沿科学研究和临床大规模筛选需求为向导,不断开发和生产出高质量的单克隆抗体和 ElISA、ELISpot、FluoroSpot试剂盒。并提供可拆分的ELISpot板和单独包装抗体等,满足了学术和工业不同需求。
更多详情可联系Mabtech中国授权一级代理商-欣博盛生物!
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