GeneTex热销内参抗体—alpha Tubulin抗体 [GT114]-深圳欣博盛生物科技有限公司

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GeneTex热销内参抗体—alpha Tubulin抗体 [GT114]

发布日期：2024-11-21

编辑：市场部

浏览次数：71

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alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.

alpha Tubulin抗体[GT114]可检测alpha Tubulin，它是一种广泛表达于细胞中的细胞骨架蛋白，通常用作 Western 印迹的负载对照。alpha Tubulin 蛋白的预测分子量为 50 kDa。这是一种被广泛引用的小鼠单克隆抗体，可用于多种应用，包括免疫印迹、免疫组织化学和免疫细胞化学。

α微管蛋白抗体[GT114]检测α微管蛋白，这是一种在细胞中广泛表达的细胞骨架蛋白，通常用作蛋白质印迹的负载对照。α-微管蛋白的预测分子量为50kDa。这是一种被广泛引用的小鼠单克隆抗体，可用于多种应用，包括蛋白质印迹、免疫组织化学和免疫细胞化学。

产品详情

产品名称：alpha Tubulin antibody [GT114]

别名：K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody

产品描述：alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.

反应种属：Human, Mouse, Rat, Zebrafish, Goat, Drosophila, Crucian carp

宿主来源：Mouse

实验应用：FACS, ICC/IF, IHC-Fr, IHC-P, WB

同种型：IgG2a

免疫原：Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

克隆性：Monoclonal

克隆号：GT114

纯化方式：Affinity purified by Protein A.

偶联：Unconjugated

产品浓度：1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度：4°C

预期分子量：50

产品形式：Liquid

存储溶液：PBS, 20% Glycerol, no Preservative.

验证数据

	WB： Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	IHC-P： alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
	ICC/IF： alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
	IHC-Fr： Immunofluorescence photomicrographs of frozen sections of mouse brain. Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04). Blue: Nuclear staining with Hoechst 33342.