GeneTex的千篇文章使用抗体 — GAPDH抗体-深圳欣博盛生物科技有限公司

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GeneTex的千篇文章使用抗体 — GAPDH抗体

发布日期：2024-11-26

编辑：市场部

浏览次数：191

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GeneTex研发的GAPDH抗体，目前有一千余篇文献引用。GAPDH抗体识别甘油醛-3-磷酸脱氢酶(GAPDH)蛋白，该蛋白是一种可以形成四聚体的约37 kDa的糖酵解酶。由于GAPDH的广泛表达，通常被用作各种蛋白质检测（尤其是 Western 印迹）的内部对照，以校正上样量，以保证实验结果的准确性。

产品详情：

产品名称：GAPDH antibody

别名：glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

产品描述：GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, a glycolytic enzyme of ~37 kDa that forms a tetramer. Because of its widespread expression, GAPDH is commonly used as an internal control for various protein assays, particularly western blot, to validate both the amount and quality of the loaded lysate samples.

反应种属：Bacteria, Bovine, Caenorhabditis elegans, Chicken, Dog, Drosophila, E. coli, Fish, Hamster, Human, Insect, milkfish, Monkey, Mosquito, Mouse, Nematode, Pig, Pika, Plant, Rabbit, Rat, Yeast, Zebrafish

宿主来源：Rabbit

实验应用：ICC/IF, IHC-P, IP, WB

同种型：IgG

免疫原：Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

克隆性：Polyclonal

纯化方式：Purified by antigen-affinity chromatography.

偶联：Unconjugated

产品浓度：0.11 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度：4°C

预期分子量：36 kDa

产品形式：Liquid

存储溶液：PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.

验证数据

	IHC-P： GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human duodenum. GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	WB： Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.
	IP： Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118). Western blot analysis was performed using GAPDH antibody (GTX100118). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
	ICC/IF： GAPDH antibody detects GAPDH protein at endoplasmic reticulum, cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).