深圳欣博盛生物科技有限公司

DR5（Death Receptor 5）是 TNF 受体超家族成员（TNFRSF10B），是 TRAIL（TNF-Related Apoptosis-Inducing Ligand，肿瘤坏死因子相关凋亡诱导配体）的死亡受体之一。TNF 家族中的某些细胞因子（包括 TNF 和 Fas Ligand）通过其含死亡结构域的受体诱导细胞凋亡。TRAIL/Apo2L 是 TNF 家族的新成员，DR4 是最早被鉴定的 TRAIL 受体，而 DR5 是随后被多个实验室独立鉴定的新型含死亡结构域的 TRAIL 受体，又称 Apo2、TRAIL-R2、TRICK2 或 KILLER。与 DR4 类似，DR5 转录本在正常组织和多种肿瘤细胞中广泛表达。DR5 结合 TRAIL 并介导 TRAIL 诱导的细胞凋亡。DR5 过表达可诱导凋亡并激活 NF-κB 通路。

DR5 Antibody（目录号 2019）是ProSci公司生产的一种兔源多克隆抗体，特异性靶向DR5（Death Receptor 5，死亡受体5），也称TRAIL-R2、TNFRSF10B或KILLER。该抗体以人DR5前体蛋白羧基末端附近20个氨基酸的肽段为免疫原制备，经肽柱亲和层析纯化。可应用于Western Blot、免疫组织化学（IHC-P）、免疫荧光（IF）及 ELISA，已在人、小鼠、大鼠样本中获得验证。该抗体与DR4无交叉反应，特异性优良，并经过基因敲除（KO）、基因敲降（KD）及调控表达等多重验证。

产品信息

实验图例

图1 KO Validation of DR5 in HCT116 Cells (Han et al., 2015)

Anti-cancer drug, Carfilzomib (CFZ), induced up-regulation of DR5 and the expression of DR5 was not detected in DR5-KO HCT 116 cell line with anti-DR5 antibodies (2019).

图2 Immunohistochemistry Validation of DR5 in Mouse kidney tissue

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (2019) at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

图3 Regulated Expression Validation of DR5 in Thyroid Epithelial Cells (Bretz et al., 2002)

Immunostaining with anti-DR5 antibodies shows high levels of DR5 expression in untreated cells and cells treated with each of the three cytokines alone or TNFalpha combined with IL-1b. In contrast, treatment with both IFNg and TNFalpha or all three cytokines greatly reduces DR5 staining. The reduction in staining appears most significant in cytoplasmic regions while some staining is maintained in or around the nucleus.

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