Bradford Protein Quantification kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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Bradford Protein Quantification kit

品牌：Bertin Bioreagent

官网链接

说明书

折扣/货期

货号：D05078.2000 dtn

规格：2000 dtn

目录价：￥2800

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Bradford Protein Quantification kit

产品描述:

The protein quantification method using Coomassie Brilliant Blue G-250 dye has been described for the first time in 1975. Three charge forms of the Coomassie Brilliant Blue dye are present in equilibrium at the usual acidic pH of the assay. The red, blue, and green forms have absorbance maxima at 470, 595, and 650 nm, respectively. The red form donates its free electron to the protein. Then the blue form interacts with the arginine, tryptophan, tyrosine, histidine and phenylalanine residues resulting in the stabilization of the blue form of the Coomassie Brilliant Blue G-250 dye. The blue anionic form is negligible at the pH of the assay reaction mixture, the binding to protein shifts the equilibrium toward its formation. The stabilized blue form of the Coomassie dye is measurable by spectrophotometry at 595 nm, the intensity of the coloration is directly linked to the amount of protein.

反应种属:

refer to technical booklet

实验应用:

Protein quantification

有效期:

Store at +4degrees; shelf life 1 years maximum after production

产地:

法国

生产商:

Bertin Bioreagent

检测范围:

0,25-5,00 µg/µL