CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag-CUTANA™ CUT&Tag-深圳欣博盛生物科技有限公司

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CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：15-1028

规格：75 µL

目录价：￥7740

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag

产品描述:

Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. CUTANA™ Uncharged 6xHis-pAG-Tn5 is a fusion of proteins A and G to E.coli transposase (Tn5), the key enzyme for CUT&Tag [1]. The fusion protein contains a polyhistidine tag (6xHis) to enable applications that require affinity purification steps (for an untagged version of uncharged pAG-Tn5, see EpiCypher 15-1025). Uncharged Tn5 must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag (see reference [1] and Application Notes). His-pAG-Tn5 enables purification of differentially barcoded antibody-Tn5 complexes such as those described in MulTI-Tag (Multiple Target Identification by Tagmentation) and multi-CUT&Tag workflows to simultaneously interrogate multiple chromatin proteins in a single CUT&Tag reaction [2,3]. These approaches have been applied to map histone post-translational modifications and RNA Polymerase II down to single cell resolution to define cell-specific epigenetic landscapes.

宿主来源:

E. coli

表达系统:

E. coli

保存温度:

Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.

运输温度:

DO NOT FREEZE!! Frozen cold packs.

产品形式:

50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

产地:

美国