| 货号:16-4201 | 规格:50 µg | 目录价:¥7140 |
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
产品名称:
EpiDyne®-FRET Nucleosome Remodeling Assay Substrate
产品描述:
This product provides a high throughput fluorescent readout of nucleosome remodeling when paired with enzymatically active remodeling complexes, such as SMARCA4, SMARCA2, and ACF (EpiCypher 15-1014, 15-1015, 15-1013). The EpiDyne®-FRET mononucleosome is assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped with a 212 base-pair DNA template bearing a 5’ Cy3, the Widom 601 nucleosome positioning element [1], and a DpnII restriction enzyme motif (GATC) that is exposed upon remodeling. H2A has a Thr to Cys substitution at residue 120, where Cy5 is conjugated. H3.2 has a Cys to Ala substitution at residue 110.
保存温度:
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产地:
美国
生产商:
EpiCypher
上一个:EpiDyne® Nucleosome Remodeling Assay Substrate
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下一个:EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1
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