H3.3 Antibody, SNAP-Certified™ for CUT&RUN-单抗-深圳欣博盛生物科技有限公司

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H3.3 Antibody, SNAP-Certified™ for CUT&RUN

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：13-0061

规格：100 µg

目录价：￥6213

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

H3.3 Antibody, SNAP-Certified™ for CUT&RUN

产品描述:

H3.3 Antibody, SNAP-Certified™ for CUT&RUN is an antibody to histone variant H3.3. H3.3 is enriched in promoters and enhancers of active genes as well as transcriptionally inactive heterochromatin, reflecting the diverse functions of this unique histone variant [1]. The antibody is highly specific for H3.3 without cross-reacting with canonical H3.1. Binding is not impacted by methylation or mutations at amino acids H3.3K27 or H3.3K36 (Figure 1). H3.3 differs from canonical H3.1 at just five amino acids (31, 87, 89, 90, 96). Most of these changes are located in the inaccessible histone core, making them unfit epitopes for chromatin mapping experiments. The SNAP-Certified™ H3.3 antibody specifically interacts with amino acid 31, where a serine is substituted for alanine, allowing clear distinction between H3.3 and H3.1 in chromatin mapping experiments. The H3.3 antibody meets EpiCypher’s SNAP-Certified™ criteria for specificity and efficiency in CUT&RUN. This requires <20% cross-reactivity to other histone variants, determined using SNAP-CUTANA™ Spike-in Controls (Figure 2). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k cells (Figure 3). Note: This antibody is sensitive to dithiothreitol (DTT).

反应种属:

Human, Wide Range (Predicted)

宿主来源:

Rabbit

实验应用:

CUT&RUN

免疫原:

A synthetic peptide corresponding to the N-terminal tail (including amino acid 31) of histone H3.3

克隆性:

Monoclonal [2893-4D5]

保存温度:

Stable for 1 year at 4°C from date of receipt