深圳欣博盛生物科技有限公司

IL-2 Superkine (Fc) [IL-2 (human):Fc (human) (rec.)]

IL-2 Superkine (human):Fc (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; Aldesleukin; Super-2

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens. IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Ralpha (CD25), IL-2Rbeta and IL-2Rgamma. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rbeta and IL-2Rgamma. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rbeta and IL-2Rgamma receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule's core that acts to stabilize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, leading to improved antitumour responses in vivo, and elicits proportionally less toxicity by lowering the expansion of Tregulatory cells and reducing pulmonary oedema.

HEK 293 cells

Binds to human and mouse IL-2R.

0.1mg/ml after reconstitution

+4°C

BI

42kDa

solid

Lyophilized. Contains PBS.

瑞士

>95% (SDS-PAGE)

Triggers T cell proliferation at concentration <10ng/ml in the presence of 250ng/mL each of anti-CD3 (ANC-144-020) and anti-CD28 (ANC-177-020) antibodies.

<0.01EU/µg protein (LAL test; Lonza).