深圳欣博盛生物科技有限公司

IL-37 (human) (monomer):Fc InVivoKine™

IL-37 (human) (monomeric):Fc Knobs-into-Holes (human) (rec.); Interleukin-1 Family Member 7; IL-1F7; FIL1 zeta; IL-1X; Interleukin-1 Homolog 4; IL-1H4; Interleukin-1 zeta; IL-1 zeta; IL-1RP1; IL1F7; FIL1Z, IL1H4, IL1RP1

IL-37 (IL-1F7; IL-1H4) is an IL-1 family member that is expressed only in certain types of human organs and cells such as heart, thymus, testis, kidney, mononuclear cells (PBMCs) and dendritic cells. IL-37 is a soluble and secreted cytokine of 218 residues that shares the beta-trefoil fold common to IL-1 family cytokines. But IL-37 is an atypical member of the IL-1 family of cytokines as it functions to inhibit many of the major pathways of innate and adaptive immunity and inflammation. It binds to the interleukin-18 receptor (IL-18R) and its co-receptor SIGIRR. IL-37 is secreted as a full-length and as a processed form starting from amino acid Val46 and assembles into an activity-limiting dimer with a dimerization constant in the nanomolar range. IL-37 mutations that lock the cytokine into its monomeric conformation (e.g. Y85A and D73K) are more active at blocking inflammation than wild-type dimer-forming IL-37 in a broad range of in vitro and in vivo activity assays. IL-37 also inhibited Lipopolysaccharide (LPS)-induced immunological reaction and LPS-induced osteoclast formation and bone resorption. IL-37 (human) (monomer):Fc InVivoKine™ (Prod. No. AG-40B-0221) is a new recombinant monomeric IL-37 produced in mammalian cells that has enhanced stability and activity compared to the classical human recombinant IL-37 produced in bacteria. This monomeric IL-37 protein is produced by using two different vectors, one encoding for the IL-37 (monomeric):Fc Knobs sequence (synthesizing a protein of 50kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-37 (human) (monomer):Fc InVivoKine™. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

HEK 293 cells

Works in human and mouse models.

After reconstitution: for 10µg size: 0.1mg/ml, for 100µg size: 1mg/ml

+4°C

BI

~55kDa and 28kDa (SDS-PAGE); ~90 kDa (Monomer) (SEC)

solid

Lyophilized. Contains PBS.

瑞士

>95% (SDS-PAGE)

<0.01EU/µg purified protein (LAL test).