MitoCheck® Complex V Activity Assay Kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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MitoCheck® Complex V Activity Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：701000

规格：96 wells

目录价：￥7356.6

产品详情

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产品名称:

MitoCheck® Complex V Activity Assay Kit

产品描述:

The mitochondrial F1FO ATP synthase, commonly referred to as complex V, is a multi-subunit protein that utilizes energy in the form of a proton gradient generated by the electron transport chain, to phosphorylate ADP to form ATP in the presence of Pi. This mechanism, referred to as oxidative phosphorylation, is responsible for ~90% of ATP generation in mammalian cells. Cayman's MitoCheck® Complex V Activity Assay measures the activity of Complex V, in the reverse reaction, as an ATPase in the mitochondrial samples provided. In this assay, ATP is dephosphorylated ADP by complex V. The ADP is then utilized by pyruvate kinase to convert phosphoenolpyruvate into pyruvate with the concomitant generation of ATP. Pyruvate, in the presence of NADH and lactate dehydrogenase, is then reduced to lactate and NAD+. The rate of NADH oxidation is monitored at 340 nm. Isolated bovine heart mitochondria are provided in this kit. Tissue mitochondria can also be acquired using Cayman’s MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).

保存温度:

-80

运输温度:

-80

有效期:

365

来源:

Animal/Bovine

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