深圳欣博盛生物科技有限公司

NDUFAB1 Antibody / NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1

NDUFAB1 antibody detects NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, encoded by the NDUFAB1 gene. Other identifiers include NADH dehydrogenase 1 alpha subcomplex protein 1, acyl carrier protein NDUFAB1, mitochondrial complex I alpha subunit 9, and human homolog of E. coli acyl carrier protein. NDUFAB1 is a mitochondrial matrix protein that plays dual roles in respiratory chain function and fatty acid biosynthesis. As part of NADH:ubiquinone oxidoreductase (complex I), it participates in the first step of the mitochondrial electron transport chain, transferring electrons from NADH to ubiquinone and contributing to proton pumping across the inner mitochondrial membrane. In addition, NDUFAB1 acts as a mitochondrial acyl carrier protein involved in fatty acid and lipoic acid synthesis, integrating energy metabolism with biosynthetic pathways.

NDUFAB1 antibody is widely applied in mitochondrial biology, metabolism, and disease research. Complex I is the largest enzyme complex of the respiratory chain, consisting of over 40 subunits, and NDUFAB1 provides both structural stability and accessory enzymatic functions. By detecting NDUFAB1, researchers can study how mitochondrial respiration couples with biosynthetic pathways to maintain cellular energy homeostasis. The dual functionality of NDUFAB1 highlights its importance beyond electron transport, as it also influences cofactor assembly and post translational modification of metabolic enzymes.

Applications of NDUFAB1 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects NDUFAB1 protein in mitochondrial fractions, immunohistochemistry maps tissue expression in high energy organs such as heart, brain, and skeletal muscle, and immunofluorescence highlights its localization in mitochondria. These methods allow researchers to connect NDUFAB1 biology to mitochondrial function at both cellular and tissue levels.

Dysfunction of NDUFAB1 or complex I assembly leads to mitochondrial disease. Deficiencies manifest as metabolic syndromes with lactic acidosis, muscle weakness, and neurodegeneration. Altered NDUFAB1 activity has been associated with Leigh syndrome, Parkinson disease, and other mitochondrial disorders. By applying NDUFAB1 antibody, scientists can study how complex I defects contribute to disease progression and explore NDUFAB1 as a diagnostic or therapeutic biomarker.

NDUFAB1 also participates in mitochondrial fatty acid synthesis. This pathway produces acyl chains used for lipoic acid synthesis, essential for pyruvate dehydrogenase and other enzyme complexes. By acting as a mitochondrial acyl carrier protein, NDUFAB1 integrates respiratory function with biosynthetic activity. Detection with antibody based assays helps researchers dissect how mitochondria coordinate catabolism and anabolism.

In cancer research, mitochondrial reprogramming is a hallmark of tumor cells. NDUFAB1 expression influences oxidative phosphorylation and biosynthetic balance, impacting proliferation and survival. Increased NDUFAB1 expression has been observed in some cancers, while reduced activity impairs energy production and cell viability. The antibody therefore provides a tool for studying mitochondrial metabolism in cancer biology.

Beyond disease, NDUFAB1 is studied in physiology, including muscle energetics, neuronal metabolism, and aging. Mitochondrial complex I function declines with age, and NDUFAB1 expression correlates with mitochondrial health. Antibody detection allows evaluation of how this subunit contributes to aging related metabolic changes. NSJ Bioreagents offers NDUFAB1 antibody with strong specificity, ensuring reliable detection of this essential mitochondrial protein across research contexts.

Human, Mouse, Rat

Rabbit

WB, IHC, IP, FACS

Rabbit IgG

A synthesized peptide derived from human NDUFAB1 was used as the immunogen for the NDUFAB1 antibody.

Recombinant Rabbit Monoclonal

30N94

Affinity-chromatography

Store the NDUFAB1 antibody at -20oC.

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA

美国