Nsp1p antibody [32D6]-单抗-深圳欣博盛生物科技有限公司

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Nsp1p antibody [32D6]

品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX24641

规格：250μl

目录价：￥5000

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Nsp1p antibody [32D6]

反应种属:

Yeast

宿主来源:

Mouse

实验应用:

ICC/IF, WB

靶标/特异性:

This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype Nsp1p is 86.5 kDa, but Nsp1p has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype Nsp1p was 108 kDa, whereas the short isoform of Nsp1p in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein Nsp1p.

同种型:

IgG

免疫原:

This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

克隆性:

Monoclonal

克隆号:

32D6

纯化方式:

Tissue culture supernatant

偶联:

Unconjugated

产品浓度:

保存温度:

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:

4°C

产品形式:

Liquid

存储溶液:

Tissue culture supernatant, 10mM Sodium azide.

生产商:

GeneTex

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