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PepMix Human CMV (pp65) - Premium Grade

Pool of 138 peptides derived from a peptide scan (15mers with 11 aa overlap) through 65 kDa phosphoprotein (pp65) (Swiss-Prot ID: P06725) of Human Cytomegalovirus (HCMV) - strain AD169.PepMix™ Human CMV (pp65) peptide pools are essential tools for studying the immune response to CMV pp65, a major Cytomegalovirus protein critical in viral pathogenesis and immune recognition. This unique peptide pool stimulates T-cell responses, targeting both CD4 and CD8 T-cells across diverse MHC contexts (refer to table). These activated T-cells typically release pro-inflammatory cytokines, including interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor (TNF), which play critical roles in modulating immune defenses against hCMV infection.PepMix™ Human CMV (pp65) - Premium Grade - SpecificationsAmount: 25 µg (approx. 15 nmol) / peptide (for stimulation of up to 2,5 x 108 cells)Purity: Premium Grade: each peptide purified to >90% (HPLC/MS) - (equivalent to an average purity of 95%)Delivery Format: Freeze-dried in glass vialApplication(s): T-cell ImmunityCondition(s)/Topic(s): Control, Opportunistic infections, Cytomegalovirus disease, InfectionStandard Delivery Time: 2-5 daysPremium Grade Quality PepMix™For applications where performance, reproducibility, and stability are critical, our new Premium Grade PepMixes™ feature multiple layers of ongoing quality control:Recurrent Performance TestingEach batch is regularly tested via ELISpot assays on two donors. Your Benefits:Assay standardization & validationMinimized performance variabilityUpdated Certificate of analysis (CoA)Cytotox TestingEach batch is tested in an in vitro Resazurin-based assay to determine if the dissolved peptides show cytotoxic effects.Endotoxin TestingPh. Eur.2.6.14A LAL test or Haemotox rFC test are performed with each batch to detect endotoxins.Sterility TestingPh. Eur.2.6.1Each batch is incubated in tryptic soy broth. Later turbidity is determined as indicator of contaminating microorganisms.Plus, our trusted quality benchmarks still apply> 90% Purity of each peptide in the pool (via HPLC-MS)Validated pooling method ensuring the presence of each peptideProduced under stringent quality managementCoA and HPLC-MS data available for each individual peptide batchAvoiding false positive T-cell responses by contaminating deletion peptidesADCF policyThe Role of CMV pp65 in hCMV Infection and Host ImmunityThe pp65 protein, a prominent tegument protein in Human Cytomegalovirus (HCMV), plays an integral role in viral replication, immune evasion, and host cell infection. As a key structural protein, it facilitates the assembly of viral particles, modulates viral gene expression, and impacts the virus-host interaction during the early stages of infection.CMV pp65 is critical in shaping the immune landscape, acting as a dominant target for both innate immunity and adaptive T-cell responses. Its ability to stimulate infected cell recognition makes it an invaluable tool for studying viral genome expression and the immune surveillance mechanisms in various cell types.PepMix™ Peptide Pool Human CMV pp65 ApplicationsThe PepMix™ Human CMV (pp65) antigen detects T-cell responsiveness to HCMV. As most people have CMV-specific T-cells recognizing HCMV pp65 the PepMix™ Human CMV (pp65) is often used as a positive control in T-cell stimulation assays. It is also useful for isolating CD4 and CD8 T-cells from PBMCs after activation. This isolation uses antibody-labeled magnetic beads. HCMV pp65-specific T-cells can be enriched through peptide stimulation and proliferation, creating T-cell lines or clones. JPT’s PepMix™ Peptide Pool Human CMV (pp65) is suitable for various cell assays needing high-quality peptides, including cytokine production-dependent assays like ELISPOT or flow cytometry, and detection of surface activation markers such as anti-CD154 or anti-CD137. Unlike non-specific controls such as as staphylococcus enterotoxin B (SEB), phorbol-12-myristate 13-acetate(PMA)/Ionomycin, or phytohemagglutinin (PHA), PepMix™ provides a T-cell receptor-dependent activation control, making it a more physiological and relevant option for peptide-based T-cell activation assays.HLA Specificty of PepMix™ Peptide Pool Human CMV pp65Peptides from the CMV pp65 protein are known to be presented by a wide range of HLA-molecules (note that some studies reported low-resolution, serologic specificities whereas others reported specific alleles). JPT can provide individual T-cell stimulating CMV-derived pp65 peptides for each mentioned allele. MHC molecules presenting peptides derived from human CMV-pp65 (see table).Applications of PepMix™ Human CMV (pp65)Detection and Functional AnalysisThe PepMix™ Human CMV (pp65) antigen is a powerful tool for:Detecting T-cell responsiveness to HCMV, a common issue during HCMV infection.Using as a positive control in T-cell stimulation assays due to its ability to stimulate CMV-specific T-cells effectively.Isolation and Enrichment of T-CellsThis product facilitates the isolation of CD4 and CD8 T-cells from PBMCs (Peripheral Blood Mononuclear Cells) through activation-dependent processes. By leveraging antibody-labeled magnetic beads, HCMV pp65-specific T-cells can be enriched and expanded, enabling the creation of T-cell lines or clones for in-depth studies.Advanced ApplicationsJPT’s PepMix™ Human CMV (pp65) is ideal for high-quality cell assays such as:Cytokine production-dependent assays, including ELISPOT and flow cytometryDetection of surface activation markers such as anti-CD154 or anti-CD137.Unlike non-specific controls like staphylococcus enterotoxin B (SEB) or phytohemagglutinin (PHA), the PepMix™ pool ensures T-cell receptor-dependent activation, providing a more physiological and reliable solution for peptide-based T-cell activation assays.Visit our webpage for more information on Peptide Tools to Study CMV.Custom Solutions for Specialized ResearchLooking for a tailored solution? Custom PepMix™ peptide pools and individual HLA-specific peptides provide flexibility for:Exploring novel host-virus interactions in hCMV infection.Standardizing T-cell assays for cross-laboratory consistency.Developing advanced tools for immune monitoring in diverse patient cohorts.PepMix™ Peptide PoolsProtein-spanning mixtures of overlapping peptides (PepMix™) are extremely efficient for- Antigen-specific T cell stimulation in T cell assays (e.g. in ELISpot, ICS, cell-mediated cytotoxicity or proliferation assays)- In-vitro T cell expansion- Dendritic cell pulsing- Immune monitoring- Cellular immune response profiling- T cell epitope identification- Standardization of T cell assaysAdvancing Research on hCMV Genes and T-cell ResponsesHarnessing the versatility of PepMix™ Human CMV pp65 enables researchers to:Decode interactions between hCMV replication and host cells.Study viral genome dynamics in infected and wild-type cells.Identify and characterize T-cell epitopes for vaccine development and therapeutic interventions.Benefits of PepMix™- Each peptide quality controlled and guaranteed for identity and purity- CoA and HPLC-MS data available- High batch-to-batch consistency- No false positive T cell responses by contaminating deletion peptides- No toxic inhibition of T cell responses due to stringent purification of each peptide- Minimization of endotoxin contamination due to low bioburden process- ADCF policy in place- HLA independent stimulation (with antigen spanning peptide pools) Unique Benefits of PepMix™ for hCMV ResearchPepMix™ peptide pools stand apart for their unmatched reliability and precision in T-cell receptor-dependent activation. Key benefits include:HLA-independent stimulation, ensuring broader applicability across MHC molecules.Superior batch-to-batch consistency for reproducible results.Minimal endotoxin contamination, preserving innate immunity response fidelity.Fully documented quality assurance, including CoA and HPLC-MS data.

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JPT Peptide Technologies

Trial Grade: each peptide purified to > 90% (HPLC/MS)