RPB1 antibody [4H8]-单抗-深圳欣博盛生物科技有限公司

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RPB1 antibody [4H8]

品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX80341

规格：50μl

目录价：￥7400

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

RPB1 antibody [4H8]

别名:

RNA polymerase II subunit A , POLR2 , POLRA , RPB1 , RPBh1 , RPO2 , RPOL2 , RpIILS , hRPB220 , hsRPB1

反应种属:

Human, Japanese Medaka

宿主来源:

Mouse

实验应用:

ChIP assay, FCM, ICC/IF, WB

靶标/特异性:

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.

同种型:

IgG1

免疫原:

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

克隆性:

Monoclonal

克隆号:

4H8

纯化方式:

Protein G purified

偶联:

Unconjugated

产品浓度:

1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:

4°C

预期分子量:

217

产品形式:

Liquid

存储溶液:

PBS, no preservatives.

生产商:

GeneTex

功能与背景:

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

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