TGR5 (GP-BAR1) Reporter Assay Kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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TGR5 (GP-BAR1) Reporter Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：601440

规格：96 wells

目录价：￥6847.4

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

TGR5 (GP-BAR1) Reporter Assay Kit

产品描述:

Cayman's TGR5 (GP-BAR1) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for expressing TGR5 and a cAMP response element regulated secreted alkaline phosphatase (SEAP) reporter (TGR5 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express TGR5 at the cell surface within 24 hours. Binding of agonists to TGR5 initiates a signal transduction cascade through the GαS and adenylate cyclase pathway resulting in the expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are collected at 6-8 hours after stimulation, and SEAP activity is measured following the addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be readily applied to high-throughput screening for therapeutic compounds regulating activation of TGR5. A selective synthetic agonist, the TGR5 Receptor Agonist (3-[2-chlorophenyl]-N-[4-chlorophenyl]-N,5-dimethyl-4-isoxazolecarboxamide), is included in the kit for use as a positive control.{27533} The kit provides sufficient reagent to measure SEAP activity at three time points using the three included white assay plates.

保存温度:

-20

运输温度:

-20

有效期:

730

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