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GeneTex高引用率冠状病毒抗体:SARS-CoV / SARS-CoV-2 (COVID-19) spike抗体 [1A9]

发布日期:2024-11-13
编辑:市场部
浏览次数:92

SARS-CoV / SARS-CoV-2 (COVID-19) spike抗体抗体能够检测SARS-CoV刺突蛋白和SARS-CoV-2刺突蛋白(S2亚基)。根据序列分析,预测该抗体也能够识别S2'亚基。经过测试表明,该抗体与MERS-CoV刺突蛋白不会产生交叉反应,还能够检测多种SARS-CoV-2 VOCs,包括奥密克戎变异株(Omicron variant)。作为GeneTex热销的冠状病毒抗体,目前该株单抗以优越的产品性能收获了300+文献引用数,而且广受使用者的好评。


产品详情

产品名称:SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

别名:2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

反应种属:SARS Coronavirus, SARS Coronavirus 2

宿主来源:Mouse

实验应用:ELISA, EM, FACS, ICC/IF, IHC-Fr, IHC-P, IHC-P (cell pellet), IP, Neutralizing/ Inhibition, Sandwich ELISA, WB

同种型:IgG1

免疫原:The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.

克隆性:Monoclonal

克隆号:1A9

纯化方式:Affinity purified by Protein G.

偶联:Unconjugated

产品浓度:1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:4°C

产品形式:Liquid

存储溶液:PBS, no preservative.

验证数据

ICC/IF实验图

ICC/IF:

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects   SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
  Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed   in 4% paraformaldehyde at RT for 30 min.
  Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2   (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

WB.png

WB:

Non-transfected (–)   and transfected (+) 293T whole cell extracts (30 μg) were separated by 10%   SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19)   spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated   anti-mouse IgG antibody (GTX213111-01) was used to detect the primary   antibody.

IP.png

IP:

Immunoprecipitation of SARS-CoV-2 Spike   transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2   (COVID-19) spike antibody [1A9] (GTX632604).
  Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19)   spike antibody [1A9] (GTX632604).
  EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to   detect the primary antibody.

IHC-P.png

IHC-P (cell pellet):

SARS-CoV /   SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2   (COVID-19) spike protein by immunohistochemical analysis.
  Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.
  Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2   (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
  Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike   antibody (GTX135356) diluted at 1:1000.
  Blue: Fluoroshield with DAPI (GTX30920).
  Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ELISA.png

ELISA:

Sandwich ELISA detection of recombinant   full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture   antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at   concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was   diluted at 1:10000 and used to detect the primary antibody.

 

产品部分引用文献

1. A Juste-Dolz et al. Anal Bioanal Chem 2024; Real-world evaluation of a QCM-based biosensor for exhaled air

实验应用:ICC/IF

2. Zhang L et al. Vaccines (Basel) 2024; 12 (5) Virological Traits of the SARS-CoV-2 BA.2.87.1 Lineage.

实验应用:WB

3. F Hornung et al. Int J Obes (Lond) 2023; Thoracic adipose tissue contributes to severe virus infection of the lung

实验应用:IHC-Fr

4. Sandra E. Reznik et al. Biomolecules 2023; 13 (8):1224 SARS-CoV-2 Infection in Unvaccinated High-Risk Pregnant Women in the Bronx, NY, USA Is Associated with Decreased Apgar Scores and Placental Villous Infarcts

实验应用:FACS

5. PS Sung et al. EMBO Mol Med 2023;:e16351 Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc

实验应用:ELISA

 

订购详情

货号

产品名称

规格

GTX632604-S

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

25 μl

GTX632604

100 μl

 


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