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GeneTex热销抗体:beta Actin抗体 [GT5512]

发布日期:2024-11-20
编辑:市场部
浏览次数:73
肌动蛋白,是真核细胞中最丰富的蛋白质,具有高度保守的序列,以单体或多聚体形式存在。肌动蛋白的多聚体形成肌动蛋白丝(fibros actin, F-actin), 可形成网络结构维持细胞的形态及构造。beta Actin是细胞骨架蛋白之一,在各组织与细胞中表达相对恒定,因此常拿来做内参。GeneTex热销抗体——beta Actin抗体,助力您的科研实验!

 

产品详情

产品名称:beta Actin antibody [GT5512]

别名:actin beta , BRWS1 , PS1TP5BP1

反应种属:Cat, Dog, Drosophila, Goat, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Yeast, Zebrafish

宿主来源:Mouse

实验应用:ICC/IF, IHC-P, IP, WB

同种型:IgG1

免疫原:Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

克隆性:Monoclonal

克隆号:GT5512

纯化方式:Affinity purified by Protein G.

偶联:Unconjugated

产品浓度:1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:4°C

预期分子量:42 kDa

产品形式:Liquid

存储溶液:PBS, no preservative.

 

验证数据

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WB Image

Beta Actin antibody [GT5512] detects   beta Actin protein by western blot analysis.

A. 20 μg 293T whole cell lysate/extract

B. 10 μg 293T whole cell lysate/extract

C. 5 μg 293T whole cell lysate/extract

D. 1 μg 293T whole cell lysate/extract

10% SDS-PAGE

Beta Actin antibody [GT5512]   (GTX629630) dilution: 1:10000

The HRP-conjugated anti-mouse IgG   antibody (GTX213111-01) was used to detect the primary antibody.

ICC.png

 ICC/IF:

beta Actin antibody [GT5512] detects   beta Actin protein at cytoskeleton by immunofluorescent analysis.

Sample: HeLa cells were fixed in 0.5%   Triton X-100 for 1 min, then ice-cold methanol for 5 min.

Green: beta Actin protein stained by   beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.

Blue: Hoechst 33342 staining.


产品部分引用文献

1.JL Bradshaw et al. Am J Physiol Regul Integr Comp Physiol 2024; Pregnancy-induced oxidative stress and inflammation are not associated with impaired maternal neuronal activity or memory function.

2.EJ Rodr?guez-Palma et al. Eur J Pharmacol 2024; 974 : 176616 Estradiol modulates the role of the spinal α6-subunit containing GABAA receptors in female rats with neuropathic pain.

3.Im JH et al. Int J Mol Sci 2024; 25 (6) Extracellular Vesicles from Cerebrospinal Fluid of Leptomeningeal Metastasis Patients Deliver MiR-21 and Induce Methotrexate Resistance in Lung Cancer Cells.

4.Lee KT et al. Elife 2024; 12 Comparative interactome analysis of α-arrestin families in human and Drosophila.


订购详情

货号

产品名称

规格

GTX629630-S

beta Actin抗体 [GT5512]

25 μl

GTX629630

100 μl

 


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