GeneTex热销标签&报告蛋白抗体：6X His tag抗体 [GT359]-深圳欣博盛生物科技有限公司

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GeneTex热销标签&报告蛋白抗体：6X His tag抗体 [GT359]

发布日期：2024-11-20

编辑：市场部

浏览次数：119

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6X His tag，也称为多组氨酸标签（polyhistidine tag），这是一种在转染的细胞中目标蛋白的C端或N端上由至少6个组氨酸残基链接上所组成的氨基酸序列，因其具有尺寸小、成本低、使用方便等优点，所以被公认为是目前使用最广泛的亲和标签。作为GeneTex热销的标签和报告蛋白抗体，6X His tag抗体一直广受好评，具有较高的文献引用率。

产品详情

产品名称：6X His tag antibody [GT359]

别名：6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

反应种属：Species independent

宿主来源：Mouse

实验应用：ELISA, ICC/IF, IP, WB

同种型：IgG3

免疫原：The immunogen used to generate this antibody corresponds to 6X His tag

克隆性：Monoclonal

克隆号：GT359

纯化方式：Affinity purified by Protein G.

偶联：Unconjugated

产品浓度：1.59 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度：4°C

产品形式：Liquid

存储溶液：PBS, 20% Glycerol, no Preservative.

验证数据

	IP： 6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] (GTX628914) 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.
	WB： 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins 15 % SDS-PAGE 6x His antibody (GTX628914) dilution: 1:5000
	IP： Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118). Western blot analysis was performed using GAPDH antibody (GTX100118). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
	ICC/IF： 6X His tag antibody [GT359] detects 6X His tag protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 6X His tag stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
	ELISA： 6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.