深圳欣博盛生物科技有限公司

表观遗传学专家EpiCypher的 CUTANA™ CUT&Tag Kit又升级啦！CUTANA™ CUT&Tag Kit升级版(V3)最大的变化是增加了一个阳性对照抗体 H3K4me3，以代表低丰度靶标，同时增加了一个 K-MetStat panel以适配这一变化，可以根据自己的实验设计选择是否使用。现在已经发布了V3版，之前的版本将不再提供哦~ 

● 促销时间：即日起 - 2025.12.24

● 促销代码：CTK20

● 促销内容：活动期间凡订购EpiCypher CUTANA™ CUT&Tag Kit，可享额外20%折扣优惠！！！

产品信息

CUTANA™ CUT&Tag Kit

CUTANA™ CUT&Tag 试剂盒为组蛋白翻译后修饰 (PTM) 的超灵敏图谱绘制提供了全面的解决方案(Kaya-Okur et al.，2019)。该试剂盒共 48 个反应，使用专有的Direct-to-PCR策略，在一个管中完成从细胞到 PCR 扩增测序文库的整个过程，跳过了传统的文库预处理，最大程度地减少了样品损失(Kaya-Okur et al.，2020)。并且与多道移液器兼容，提高了通量和可重复性。阳性(H3K27me3 和 H3K4me3)和阴性(IgG)对照抗体与 SNAP-CUTANA™ K-MetStat Panel 核糖体掺入对照(图2)配对，以持续监控实验流程并指导故障排除。

CUT&Tag的推荐输入量是每个反应100,000个原生核。可比较的数据低至10,000个细胞核，并且该方案也可用于整个细胞、冷冻保存样本和轻度交联的细胞核或细胞进行验证。CUT&Tag为组蛋白 PTMs 提供了强大的分析功能。对于染色质相关蛋白(如转录因子)，建议使用CUTANATM CUT&RUN (EpiCypher 14-1048，EpiCypher 14-1001/14-1002)。

◉ 验证数据

	Figure 1: CUT&Tag DNA fragment size distribution analysis
	CUT&Tag was performed as described in Figure 5. Library DNA was analyzed by Agilent TapeStation®, which confirmed that mononucleosomes were predominantly enriched in CUT&Tag (peak between 300-400 bp). Peak between 500-700 bp represents dinucleosomes.
	Figure 2: SNAP-CUTANA™ K-MetStat Spike-in controls
	DNA-barcoded designer nucleosomes (dNucs) representing 16 different K-methyl PTM states: mono-, di-, and tri-methylation at H3K4, H3K9, H3K27, H3K36, and H4K20, as well as unmodified control, were spiked into CUT&Tag samples prior to the addition of the control antibodies provided with the kit (IgG, H3K27me3, H3K4me3). After sequencing, instances of each spike-in barcode recovered in the CUT&Tag reactions were counted and normalized from raw fastq files using the shell script and analysis Excel sheet available on the spike-in product page (EpiCypher 19-1002). Barcodes for IgG (top; normalized to the sum of total reads), H3K27me3 (middle; normalized to on-target), and H3K4me3 (bottom; normalized to on-target) antibodies provided with this kit are shown. The spike-ins confirmed optimal experimental conditions (H3K27me3 and H3K4me3 antibodies specifically recovered the target dNuc, while IgG showed no preferential enrichment).
	Figure 3: CUT&Tag genome-wide heatmaps
	CUT&Tag was performed as described in Figure 5. Heatmaps show two replicates (“Rep”) of IgG and H3K4me3 antibodies in aligned rows ranked by intensity (top to bottom) relative to the H3K4me3 Rep 1 reaction. High, medium, and low intensity are shown in red, yellow, and blue, respectively. Antibodies to histone PTMs showed expected enrichment patterns and high reproducibility. H3K4me3, a marker of active transcription localized to transcription start sites (TSSs), shows enrichment consistent at TSSs, as expected. IgG shows low background enrichment.
	Figure 4: Representative gene browser tracks
	CUT&Tag was performed as described in Figure 5. A representative 186 kb window at the LAMC3 gene is shown for two replicates (“Rep”) of IgG, H3K27me3, and H3K4me3 kit control antibodies. Representative tracks are also shown for two replicates of H3K4me1 antibody. The CUT&Tag kit produced the expected genomic distribution for each target. Images were generated using the Integrative Genomics Viewer (IGV, Broad Institute).
	Figure 5: CUT&Tag methods
	CUT&Tag was performed using the CUTANA™ CUT&Tag Kit starting with 100k K562 cells and 0.5µg of either IgG (EpiCypher 13-0042t), H3K27me3 (EpiCypher 13-0055t), H3K4me3 (EpiCypher 13-0060t), or H3K4me1 (EpiCypher 13-0057) antibodies. Libraries were run on an Illumina NextSeq2000 with paired-end sequencing (2x50 bp). Sample sequencing depth was 5.3/4.1 million reads (IgG Rep 1/Rep 2), 11.2/9.0 million reads (H3K27me3 Rep 1/Rep 2), 8.6/5.0 million reads (H3K4me3 Rep 1/Rep 2), and 4.1/10.3 million reads (H3K4me1 Rep 1/Rep 2). Data were aligned to the hg19 genome using Bowtie2. Data were filtered to remove duplicates, multi-aligned reads, and ENCODE DAC Exclusion List regions.

◉ 产品组分 

◉ 参考文献

Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5. PMID: 31036827; PMCID: PMC6488672.

Kaya-Okur HS, Janssens DH, Henikoff JG, Ahmad K, Henikoff S. Efficient low-cost chromatin profiling with CUT&Tag. Nat Protoc. 2020 Oct;15(10):3264-3283. doi: 10.1038/s41596-020-0373-x. Epub 2020 Sep 10. PMID: 32913232; PMCID: PMC8318778.

◉ 关于EpiCypher公司：

EpiCypher是一家成立于2012年的表观遗传学公司。从专有组蛋白肽阵列平台EpiGold™开始，EpiCypher开发了一系列同类产品。同时，EpiCypher是重组核小体制造和开发的全球领导者。利用其独有技术，不断增加产品库中高纯度修饰重组核小体(dNucs™)产品。dNuc™多样性的产品为破译组蛋白编码和加速药物开发提供了强大的工具。

EpiCypher还将dNuc™技术广泛的应用于多种分析测定产品中，包括：SNAP-ChIP^®Spike-in Controls(用于抗体分析和ChIP定量)， EpiDyne^®底物(用于染色质重塑和抑制剂筛选及开发)，dCyher™测定(用于探究表观遗传蛋白质-组蛋白PTM结合相互作用)。最近，EpiCypher还推出了针对ChIC、CUT&RUN和CUT&Tag的高灵敏度表观基因组图谱CUTANA™分析。

如需了解更多详细信息或相关产品，请联系EpiCypher中国授权代理商-欣博盛生物 

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