深圳欣博盛生物科技有限公司

CUTANA™ Fiber-seq Kit

The CUTANA™ Fiber-seq Kit provides key reagents and a comprehensive manual to perform Fiber-seq. Key advantages include: • Multiomic insights in one assay – Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants, consolidating what typically requires three or more separate assays into one long-read sequencing (LRS) assay [1]. • Comprehensive solution – This kit includes key reagents needed to go from cells to library prep-ready DNA. • User-friendly resources – The User Manual is complete with a detailed protocol, QC checks, FAQs, and expert tips to get you started, while the bench-proof Quick-Start Card provides a concise protocol for convenient reference. • Easy workflow integration – Fiber-seq can be performed in <2 hours directly upstream of routine long-read sequencing library preparation and uses reagents vetted for compatibility with library prep kits from major LRS platforms. • Powered by Hia5 6mA methyltransferase – Hia5 is the highest activity 6mA-methyltransferase available and is exclusive to CUTANA Fiber-seq Kits. This more active enzyme enables faster incubations, which results in better resolution of protein footprints [2-4]. • Stringent quality control – Each new lot undergoes rigorous quality control checks to ensure components yield consistent results in your research. Fiber-seq is a breakthrough approach that simultaneously maps genetic and epigenetic features from individual chromatin fibers. This single assay delivers insights that would normally require integrating data from multiple methods such as ATAC-seq, ChIP-seq, and bisulfite sequencing. This kit includes reagents to go from cells to purified genomic DNA that can be used directly in either Pacific Biosciences® (PacBio® HiFi Sequencing) or Oxford Nanopore Technologies® (ONT® Nanopore Sequencing) library preparation workflows. The recommended input for Fiber-seq is 1,000,000 native human nuclei per reaction. The Fiber-seq Kit has been successfully applied to cryopreserved and lightly cross-linked nuclei (refer to the Tech Support Center at support.epicypher.com for the most up-to-date recommendations).

OPEN KIT IMMEDIATELY and store components at room temperature, 4°C, -20°C, and -80°C as indicated (see Kit Version 1 User Manual). Stable for 6 months upon date of receipt.

Frozen cold packs.

美国

EpiCypher