InvivoGen 细胞因子报告细胞系
我们的HEK-BlueTM细胞因子报告细胞作为一个不断拓展的工程化细胞系系列，旨在提供一种简单快速且可靠的方法来监测关键细胞因子诱导的信号通路激活。每个批次都经过功能测试和验证，以确保性能的一致性。每个HEK-BlueTM细胞因子报告细胞系都有一个分泌型胚胎碱性磷酸酶SEAP（secreted embryonic alkaline phosphatase）报告基因，该基因受所研究细胞因子诱导的转录因子调控。使用Quanti-BlueTM Solution可以轻松评估SEAP活性。这些细胞能灵敏监测具有生物活性的细胞因子，是筛选具有激动剂或拮抗剂活性的化合物（如中和抗体）的理想选择。
主要特点
• HEK293衍生的工程化细胞系
• 易于比色读数和快速定量
• 对目标细胞因子具有高灵敏度
• 经细胞因子特异性测试
• 经认证不含支原体
• 至少可稳定传代 20 次
应 用
• 开发治疗方案
• 药物筛选
• 释放试验
🔍 每种产品的所有数据均可在我们的网站上找到。
| 产品 | 检测到的细胞因子/通路 | 报告基因 | 货号 |
| 干扰素报告细胞 | |||
| HEK-Blue™ IFN-α/β | Human&mouse type I IFNs/STAT1-STAT2-IRF9 (ISGF3) | SEAP | hkb-ifnabv2 |
| HEK-Blue™ IFN-γ | Human type II IFN/STAT1 | SEAP | hkb-ifng |
| HEK-Blue™ IFN-λ | Human&mouse type III IFNs (IL-28a, IL-28b, IL-29)/STAT1-STAT2-IRF9(ISGF3) | SEAP | hkb-ifnlv2 |
| 白介素报告细胞 | |||
| HEK-Blue™ IL-1β | Human&mouse IL-1α/β, knock-out of TNFR1/NF-κB | SEAP | hkb-il1bv2 |
| HEK-Blue™ IL-1R | Human&mouse IL-1α/β/NF-κB | SEAP | hkb-il1r |
| HEK-Blue™ IL-2/IL-15 | Human&mouse IL-2, human IL-15/STAT5 | SEAP | hkb-il2 |
| HEK-Blue™ CD122/CD132 | Human&mouse IL-2, human IL-15/STAT5 | SEAP | hkb-il2bg |
| HEK-Blue™ IL-3 | Human IL-3/STAT5 | SEAP | hkb-il3 |
| HEK-Blue™ IL-4/IL-13 | Human IL-4 and human&mouse IL-13/STAT6 | SEAP | hkb-il413 |
| HEK-Blue™ IL-5 | Human&mouse IL-5/STAT5 | SEAP | hkb-il5 |
| HEK-Blue™ IL-6 | Human IL-6/STAT3 | SEAP | hkb-hil6 |
| HEK-Blue™ IL-7 | Human&mouse IL-7/STAT5 | SEAP | hkb-il7 |
| HEK-Blue™ IL-9 | Human&mouse IL-9/STAT5 | SEAP | hkb-il9 |
| HEK-Blue™ IL-10 | Human&mouse IL-10/STAT3 | SEAP | hkb-il10v2 |
| HEK-Blue™ IL-11 | Human&mouse IL-11/STAT3 | SEAP | hkb-hil11rv2 |
| HEK-Blue™ IL-12 | Human&mouse IL-12/STAT4 | SEAP | hkb-il12 |
| HEK-Blue-GFP™ IL-12 | Human&mouse IL-12/STAT4 | SEAP/GFP | hkbg-il12 |
| HEK-Blue™ IL-17 | Human IL-17A/F and human&mouse IL-17E/NF-κB | SEAP | hkb-il17 |
| HEK-Blue™ IL-17C | Human&mouse IL-17C/E and human IL-17A/NF-κB | SEAP | hkb-il17c |
| HEK-Blue™ IL-18 | Human&mouse IL-18/NF-κB | SEAP | hkb-hmil18 |
| HEK-Blue™ IL-19/IL-20 | Human&mouse IL-19, IL-20&IL-24/STAT3 | SEAP | hkb-il1920 |
| HEK-Blue™ IL-20 | Human&mouse IL-20 IL-22&IL-24/STAT3 | SEAP | hkb-il20 |
| HEK-Blue™ IL-21 | Human&mouse IL-21/STAT3 | SEAP | hkb-il21 |
| HEK-Blue™ IL-22 | Human&mouse IL-22/STAT3 | SEAP | hkb-il22 |
| HEK-Blue™ IL-23 | Human&mouse IL-23/STAT3 | SEAP | hkb-il23 |
| HEK-Blue™ IL-31 | Human IL-31/STAT5 | SEAP | hkb-il31 |
| HEK-Blue™ IL-33 | Human&mouse IL-33/NF-κB | SEAP | hkb-hil33 |
| HEK-Blue™ IL-36 | Human IL-36/NF-κB | SEAP | hkb-hil36r |
| 生长因子、集落刺激因子和类激素报告细胞 | |||
| HEK-Blue™ EPO | Human&mouse EPO/STAT5 | SEAP | hkb-epo |
| HEK-Blue™ G-CSF | Human&mouse GM-CSF/STAT3 | SEAP | hkb-gcsf |
| HEK-Blue™ GM-CSF | Human GM-CSF/STAT5 | SEAP | hkb-hgmcsfr |
| HEK-Blue™ GLP-1 | Human GLP1/CREB | SEAP | hkb-glp1 |
| HEK-Blue™ TGF-β | Human&mouse TGF-β/Smad | SEAP | hkb-tgfb |
| HEK-Blue™ TPO | Human&mouse TPO/STAT5 | SEAP | hkb-tpo |
| HEK-Blue™ TSLP | Human TSLP/STAT5 | SEAP | hkb-tslp |
| 肿瘤坏死因子相关报告细胞 | |||
| HEK-Blue™ BAFF-R | Human&mouse BAFF/NF-κB | SEAP | hkb-baffr |
| HEK-Blue™ BCMA | Human&mouse APRIL, human&mouse BAFF/NF-κB | SEAP | hkb-bcma |
| HEK-Blue™ CD40L | Human&mouse CD40L/NF-κB | SEAP | hkb-cd40 |
| HEK-Blue™ RANKL | Human&mouse RANKL/NF-κB | SEAP | hkb-ranklv2 |
| HEK-Blue™ TACI | Human&mouse APRIL, human&mouse BAFF/NF-κB | SEAP | hkb-taci |
| HEK-Blue™ TNF-α | Human&mouse TNF-α/NF-κB | SEAP | hkb-tnfdmyd |
| HEK-Blue™ TL1A | Human&mouse TL1A/NF-κB | SEAP | hkd-tl1a |
| 特色产品 | HEK-BLUE™ TSLP CELLS |
Signaling pathway in HEK-BlueTM TSLP cells |
(A) HEK-Blue™ TSLP cells were stimulated with increasing concentrations of human (h) or mouse (m) TSLP. (B) Increasing concentrations of Anti-hTSLP-hIgG2 (0.3 ng/ml - 30μg/ml) were incubated with hTSLP (1ng/ml) for 1 h before the addition of HEK-Blue™ TSLP cells. (C) HEK-Blue™ TSLP cells were stimulated with various human and mouse recombinant cytokines:10ng/ml of hTSLP, mTSLP, hIFN-γ, hTNF-α, hIL-1β, hIL-2, hIL-7, hIL-9, hIL-15, and 1000 U/ml hIFN-α. After overnight incubation, SEAP activity was assessed using QUANTI‑Blue™ Solution. The optical density (OD) at 650 nm is shown as mean ± SEM. |
 | 细胞因子优惠 |  | Additional cell vial | |
| 每购买一只细胞因子报告细胞系, 即可免费获赠一瓶相应的细胞因子。 | 购买相同细胞时，第二支起可享优惠。 详情请参阅网站上的条款。 | |||
| 详情请参阅网站上的条款。 |  | invivogen.com/cytokine-reporter-cells | ||
相关产品 | 货号 |
QUANTI-Blue™ Solution | rep-qbs |
Normocin® | ant-nr-1 |
Blasticidin | ant-bl-05 |
Zeocin® | ant-zn-05 |
Puromycin | ant-pr-1 |
HEK-Blue™ Selection | hb-sel |
更多详情请联系 InvivoGen 中国授权代理-欣博盛生物
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