CUTANA™ Multiomic CUT&RUN Controls Set-其他表观遗传研究工具-深圳欣博盛生物科技有限公司

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CUTANA™ Multiomic CUT&RUN Controls Set

品牌：EpiCypher

官网链接

说明书

折扣/货期

货号：14-1802

规格：1 Set

目录价：￥2700

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

CUTANA™ Multiomic CUT&RUN Controls Set

产品描述:

The CUTANA™ Multiomic CUT&RUN Controls Set is a specialized set of controls designed to be paired with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) to enable direct, simultaneous analysis of DNA methylation and chromatin proteins in a single workflow. Following isolation of chromatin protein-bound DNA in CUT&RUN, DNA fragments are prepared for sequencing with a cytosine conversion strategy such as Enzymatic Methyl-seq (NEB® EM-seq™, preferred) or bisulfite sequencing to provide base-pair resolution of 5-methylcytosine (5mC). This set includes the essential controls for CUT&RUN followed by EM-seq (CUT&RUN-EM). H3K36me3 antibody is a positive control that enriches DNA associated with active gene bodies, which contain high levels of DNA methylation. H3K36me3 antibody complements the antibodies included in the CUT&RUN kit (H3K4me3, which enriches unmethylated promoters, and IgG, which serves as a negative control) to validate the technical success of the experimental workflow. Pre-fragmented methylated pUC19 and unmethylated Lambda DNAs serve as controls in EM-seq to assess conversion efficiency of unmethylated cytosines. This controls set is a tailored solution to unlock the capacity of CUT&RUN to generate multiomic insights and investigate crosstalk between DNA methylation and chromatin proteins.

保存温度:

OPEN SET IMMEDIATELY and store components at room temperature, 4°C, and -20°C as indicated. Stable for 6 months upon date of receipt.

运输温度:

DO NOT FREEZE!! Frozen cold packs.

产地:

美国

生产商:

EpiCypher

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