| 货号:21-1026 | 规格:100 Reactions | 目录价:¥3720 |
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
产品名称:
CUTANA™ Nuclei Extraction Buffer
产品描述:
CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer:
mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells
human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells)
human bone marrow derived macrophages human TIG-1 fetal lung cells
human monocyte derived macrophages human LoVo colorectal cancer cells
human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells
human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells
human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs)
human MCF7 breast cancer cells human intestinal tissue
human MDA-MB-231 breast cancer cells human HEPM embryonic cells
human SK-MEL-2 melanoma cells
The reagent is prepared by supplementing Pre-Nuclei Extraction Buffer with protease inhibitor and spermidine fresh on the day of use. Utilize this buffer in EpiCypher’s CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag (epicypher.com/protocols) to obtain the highest quality nuclei for your genomic mapping assay.
实验应用:
CUT&RUN, CUT&Tag
保存温度:
Store the Pre-Nuclei Extraction Buffer at 4ºC and the 1 M Spermidine at -20ºC. Components are stable for 6 months upon date of receipt.
运输温度:
DO NOT FREEZE!! Frozen cold packs.
产品形式:
Protease inhibitor is not included. EpiCypher suggests using CUTANA™ Protease Inhibitor Tablets (EpiCypher 21-1027).
产地:
美国
生产商:
EpiCypher
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