HSP70 Antibody: APC-单抗-深圳欣博盛生物科技有限公司

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HSP70 Antibody: APC

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SMC-249D-APC

规格：100 µg

目录价：￥5707.8

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

HSP70 Antibody: APC

别名:

HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody

产品描述:

Mouse Anti-Human Cell Membrane HSP70 Monoclonal IgG1

反应种属:

Human | Mouse | Rat | Bovine | Pig

宿主来源:

Mouse

实验应用:

WB | ICC/IF | FCM | FACS

靶标/特异性:

Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa band in Western Blot.

同种型:

IgG1

推荐稀释度:

WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.

免疫原:

Human native HSP70 protein

免疫原种属:

Human

克隆性:

Monoclonal

克隆号:

1H11

纯化方式:

Protein G Purified

偶联:

APC

产品浓度:

0.5 mg/ml

保存温度:

Conjugated antibodies should be stored according to the product label

运输温度:

Blue Ice or 4ºC

存储溶液:

PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated

产地:

加拿大

功能与背景:

HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.

Accession #:

NP_005336.3