Human Recombinant Alpha Synuclein S87N Mutant Pre-formed Fibrils-其他重组蛋白-深圳欣博盛生物科技有限公司

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Human Recombinant Alpha Synuclein S87N Mutant Pre-formed Fibrils

品牌：Stressmarq

官网链接

说明书

折扣/货期

货号：SPR-500XE

规格：500 µg (@5mg/ml)

目录价：￥18333

产品详情

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产品名称:

Human Recombinant Alpha Synuclein S87N Mutant Pre-formed Fibrils

别名:

MAPT, 2N4R, Tau40 neurofibrillary tangle protein, paired-helical filament, PHFs, SNCA, NACP, PARK1, asyn, alpha-synuclein, pre-formed fibril, PFFs, mixed fibrils

产品描述:

Human Recombinant Alpha Synuclein S87N Mutant Pre-formed Fibrils

反应种属:

Human

实验应用:

WB | In vivo Assay | In vitro Assay

纯化方式:

Ion-exchange Purified

偶联:

No Tag

产品浓度:

2 mg/ml or 5 mg/ml

表达系统:

E. coli

保存温度:

-80ºC

运输温度:

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

预期分子量:

14.46 kDa

存储溶液:

1X PBS pH 7.4

产地:

加拿大

功能与背景:

Human alpha synuclein S87N mutant (HuS87N) has Ser87 mutated to the equivalent mouse residue Asn87, effectively making it a human-mouse chimeric protein. Despite sequence differences at only seven residues, or 5% of the total 140 amino acids, the aggregation rate of wild-type mouse α-syn (MsWT) is faster than wild-type human α-syn (HuWT) in vitro. In wild-type mouse models, MsWT fibrils are more efficient than HuWT fibrils at inducing endogenous mouse α-syn pathology (1). A53T or S87N substitutions in human α-syn substantially accelerate fibrilization rates in vitro (2,3). Chimeric HuS87N fibrils show enhanced induction of α-syn pathology greater than both HuWT and MsWT fibrils in mice neuron cultures (4). Therefore, HuS87N is a good construct for inducing robust endogenous α-syn seeding and pathology in wild-type mice/cultures.

纯度:

>95%

Accession #:

NP_000336.1