深圳欣博盛生物科技有限公司

IL-4 (mouse) (monomer):Fc (silent) InVivoKine™

InVivoKine™; IL-4 (mouse) (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interleukin-4; B Cell Stimulatory Factor 1; BSF-1; IGG1 Induction Factor

Interleukin-4 (IL-4) is a monomeric TH2 cytokine of approximately 17kDa that shows pleiotropic effects during immune responses. It functions as a potent regulator of immunity. IL-4 was first identified as a T cell-derived growth factor for B cells. IL-4 and IL-13 activate many common signaling pathways. IL-4 is mainly produced by Th2 cells, but can also be secreted by natural killer T (NKT) cells, mast cells, eosinophils, basophils and innate-type lymphoid cells. IL-4 can signal through the type II IL-4 receptor, which is a heterodimer of IL-4Ralpha and IL-13Ralpha1 chains, and also through the type I IL-4 receptor, which is a heterodimer of IL-4 receptor alpha (IL-4Ralpha) and common gamma (gammac) chains. IL-4 binding results in tyrosine kinase Janus kinase 1 (JAK1) and JAK3 activation. IL-4 and IL-13 may be involved at several levels of the fibrotic process since they activate the fibroblasts that differentiate into myofibroblasts and they stimulate extracellular matrix (ECM) production and deposition. IL-4 functions as a co-mitogen of B-cells. It is important in leukocyte survival, Th2 cell-mediated immunity, IgE class switching in B cells and tissue repair and homeostasis through 'alternative' macrophage activation. The IL-4 (mouse) (monomer):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IL-4 (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 50-55kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-4 (mouse) (monomer):Fc (silent) InVivoKine™. The LALA-PG mutations inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

HEK 293 cells. Produced using animal component-free medium.

The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcgammaRs and therefore Fc undesirable effects.

After reconstitution: 1mg/ml

+4°C

BI

~50-55kDa and 28kDa (SDS-PAGE)

solid

Lyophilized. Contains PBS.

瑞士

>95% (SDS-PAGE)

<0.01 EU/µg protein (LAL test).