| 货号:700950 | 规格:96 wells | 目录价:¥6592.8 |
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
产品名称:
MitoCheck® Complex II/III Activity Assay Kit
产品描述:
Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone. Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle. During the Q-cycle, electrons are passed from QH2 onto cytochrome c via the Rieske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-. Cayman's MitoCheck® Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III. This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2. Due to the dependence on complex II activity, a counter-screen for complex II activity (Item No. 700940) should be performed in order to truly measure complex III activity.
保存温度:
-80
运输温度:
-80
有效期:
730
来源:
Animal/Bovine|Animal/Horse
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