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折扣/货期| 货号：16-0350 | 规格：50 µg | 目录价：￥5700 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
Mononucleosomes (H3.3K9M), Recombinant Human Biotinylated
产品描述:
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.3-P84243, H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA, identified by Lowary and Widom [1]. H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3) contains a lysine-to-methionine (K-to-M) substitution at position nine. K-to-M oncohistone mutations are known to be prevalent in cancer and to affect chromatin regulatory mechanisms(Nacev et al., 2018). The 601 sequence DNA contains a 5’ biotin-TEG group, enabling affinity binding applications.
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
H3.3K9M mononucleosomes (27.3 µg protein weight, 50 µg DNA + protein) in 50.4 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
Molarity = 4.95 μM. MW = 200,543.
产地:
美国
生产商:
EpiCypher
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下一个:H3.3G34R Mononucleosome, Biotinylated
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