Myeloperoxidase Chlorination and Peroxidation Inhibitor Screening Assay Kit
| 货号:702930 | 规格:2 x 96 wells | 目录价:¥5386.8 |
产品详情
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产品名称:
Myeloperoxidase Chlorination and Peroxidation Inhibitor Screening Assay Kit
别名:
Myeloperoxidase
产品描述:
Cayman’s MPO Inhibitor Screening Assay Kit provides convenient fluorometric methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes a non-fluorescent thiomorphiline-based probe (NBD-TM), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound, NBD.{71274} Fluorescence intensity is analyzed using an excitation wavelength of 500 nm and an emision wavelength of 550 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two-electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one-electron reductions back to native enzyme by compound II (MPO-II).{16905} The reaction between hydrogen peroxide and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.
保存温度:
-20
运输温度:
-20
有效期:
365
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