Orexin 1 Receptor Reporter Assay Kit-比色法检测试剂盒-深圳欣博盛生物科技有限公司

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Orexin 1 Receptor Reporter Assay Kit

品牌：Cayman

官网链接

说明书

折扣/货期

货号：600240

规格：100 tests

目录价：￥6847.4

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Orexin 1 Receptor Reporter Assay Kit

别名:

OX1R

产品描述:

Cayman's OX1R Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for OX1R, a recombinant G-protein, and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (OX1R Reverse Transfection Strip Plate). Cells grown on the transfection complex will express OX1R at the cell surface and the recombinant G-protein inside the cell. Binding of agonists to OX1R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are removed at time intervals beginning at about 16 hours and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of OX1R. A known OX1R agonist, Orexin A, is included in the kit for use as a positive control. The kit provides sufficient reagent to measure SEAP activity at three time points, using the black plates provided.

保存温度:

-80

运输温度:

-80

有效期:

730

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