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折扣/货期| 货号：19-1001 | 规格：20 µL | 目录价：￥4380 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
SNAP-ChIP® K-MetStat™ Panel
产品描述:
A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431 or H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The mononucleosomes constitute a pool of 1 unmodified plus 15 histone H3 or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4, K9, K27, and H4K20 with me1, me2, or me3. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA (“barcode”) at the 3’ end that can be deciphered by qPCR or next-generation sequencing. Each of the 16 nucleosomes in the pool is wrapped by 2 distinct DNA species, each containing a unique barcode (”A” and “B”, see SNAP-ChIP Manual) allowing for an internal technical replicate. * Histone H3.2 contains a Cys to Ala substitution at position 110.
保存温度:
Stable for six months at -20°C from date of receipt.
运输温度:
DO NOT FREEZE!! Frozen cold packs.
产品形式:
Purified recombinant mononucleosomes, containing a mixture of 16 (1 unmodified plus 15 unique) H3 and H4 PTMs in 10 mM sodium cacodylate pH 7.5, 100 mM NaCl, 1 mM EDTA, 50% glycerol (w/v), 1x Protease Inhibitor cocktail, 100 µg/mL BSA, 10 mM β-mercaptoethanol. Average molarity = 0.6 nM.
MW = ~199382.1 Da (average MW of all 16 nucleosomes).
产地:
美国
生产商:
EpiCypher
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