官网链接
折扣/货期| 货号：19-1002 | 规格：50 Reactions | 目录价：￥3540 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
SNAP-CUTANA™ K-MetStat Panel
产品描述:
The SNAP-CUTANA™ K-MetStat Panel of spike-in controls for CUT&RUN and CUT&Tag offers an all-in-one solution to determine antibody specificity for histone post-translational modifications (PTMs), monitor assay success, and normalize data for quantitative chromatin mapping. The panel contains designer nucleosomes (dNucs) representing 15 different K-methyl PTM states: mono-, di-, and trimethylation at H3K4, H3K9, H3K27, H3K36, & H4K20, as well as unmodified control (Figure 1). Each PTM is represented by two unique DNA-barcoded templates (A and B, for an internal technical replicate). Each dNuc is individually conjugated to paramagnetic beads and pooled into a single panel for convenient one-step spike-in to CUT&RUN and CUT&Tag experiments. The panel is added to ConA-immobilized cells prior to the addition of an antibody targeting a histone lysine methylation state or IgG negative control (see Application Notes and Table 1). pAG-MNase-mediated release or pAG-Tn5-mediated tagmentation of genomic chromatin and the barcoded nucleosomes is dependent on the specificity of the antibody used. After sequencing, the relative read count of each spike-in nucleosome barcode provides a quantitative metric of on- vs. off-target recovery (Figures 4 and 5) as well as quantitative sample normalization, thereby gauging experimental success, guiding troubleshooting efforts, and enabling reliable cross-sample comparisons.
实验应用:
This panel is compatible with our CUT&RUN kits (EpiCypher, 14-1048) and DIY CUT&RUN and CUT&Tag protocols (epicypher.com/protocols). For detailed instructions on integrating spike-ins to CUT&RUN/ CUT&Tag workflows, see our most recent user guide at:
epicypher.com/19-1002. For a brief overview, see next page.
保存温度:
Store at -20°C. Lower temperatures can cause freezing and will permanently damage the magnetic beads. Stable for six months from date of receipt.
Pipette to resuspend beads; DO NOT VORTEX.
运输温度:
Frozen cold packs. DO NOT FREEZE.
产品形式:
A mixture of 16 PTM-defined semi-synthetic nucleosomes conjugated to paramagnetic beads in 10 mM sodium cacodylate pH 7.5, 100 mM NaCl, 1 mM EDTA, 50% glycerol (w/v), 1x Protease Inhibitor Cocktail, 100 μg/mL BSA, 10 mM β-mercaptoethanol.
产地:
美国
生产商:
EpiCypher
您可能感兴趣的产品
SNAP-ChIP® K-MetStat™ Panel
货号：19-1001
SNAP-ChIP® OncoStat™ Panel
货号：19-2001
SNAP-ChIP® K-MetStat™ Panel
货号：19-1100
SNAP-ChIP® OncoStat™ Panel
货号：19-2100
SNAP-ChIP® K-AcylStat™ Panel
货号：19-3001
dCypher™ Nucleosome Full Panel
货号：16-9001
SNAP-ChIP® K-AcylStat™ Panel
货号：19-3100
SNAP-CUTANA™ K-MetStat Panel
货号：19-1002
促销活动
精品推荐
