Thymine Dimer antibody [H3]-单抗-深圳欣博盛生物科技有限公司

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Thymine Dimer antibody [H3]

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品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX10347

规格：25μl

目录价：￥5300

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Thymine Dimer antibody [H3]

反应种属:

Species independent

宿主来源:

Mouse

实验应用:

Dot, ELISA, ICC/IF, WB

同种型:

IgG1

免疫原:

tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.[1]

克隆性:

Monoclonal

克隆号:

纯化方式:

Purified immunoglobulin

偶联:

Unconjugated

产品浓度:

~2 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:

4°C

产品形式:

Liquid

存储溶液:

PBS, 15mM Sodium azide.

生产商:

GeneTex

功能与背景:

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

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