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折扣/货期| 货号：CDX-D0156-G001 | 规格：1 g | 目录价：￥434 |
| 货号：CDX-D0156-G005 | 规格：5 g | 目录价：￥980 |
| 货号：CDX-D0156-G025 | 规格：25 g | 目录价：￥2226 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
DTNB
别名:
Ellman's Reagent; 5,5′-Dithiobis(2-nitrobenzoic acid)
产品描述:
A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.
宿主来源:
Synthetic
保存温度:
+20°C
运输温度:
Ambient
预期分子量:
396.35
产品形式:
solid
有效期:
Stable for at least 2 years after receipt when stored at +20°C.
产地:
瑞士
纯度:
>98% (Titration)
CAS号:
69-78-3
分子式:
C14H8N2O8S2
溶解度:
Soluble in ethanol, dioxane or phosphate buffer.
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