Jurkat whole cell lysate-细胞和组织裂解液-深圳欣博盛生物科技有限公司

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Jurkat whole cell lysate

品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX30596

规格：150μl

目录价：￥询价

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

Jurkat whole cell lysate

产品描述:

Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na₃VO₄ were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

产品浓度:

1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

运输温度:

dry ice

产品形式:

Liquid

存储溶液:

1x SDS sample buffer, 5% β-mercaptoethanol, no preservative.

生产商:

GeneTex