HL-60 whole cell lysate-细胞和组织裂解液-深圳欣博盛生物科技有限公司

首页产品中心细胞和组织裂解液

HL-60 whole cell lysate

品牌：GeneTex

官网链接

说明书

折扣/货期

货号：GTX05057

规格：500μg

目录价：￥询价

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

HL-60 whole cell lysate

产品描述:

Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na₃VO₄ were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/ml with 1X RIPA buffer. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95º C for 5 minutes.

偶联:

Unconjugated

产品浓度:

4 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

This whole cell lysate is stable for three months at -20ºC

运输温度:

Dry ice

产品形式:

Liquid

存储溶液:

1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors, no preservatives.