A549 whole cell lysate-细胞和组织裂解液-深圳欣博盛生物科技有限公司

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A549 whole cell lysate

品牌：GeneTex

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说明书

折扣/货期

货号：GTX05055

规格：500μg

目录价：￥询价

产品详情

* 以下信息仅供参考，详情请以原厂网站为准

产品名称:

A549 whole cell lysate

产品描述:

Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na₃VO₄ were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).

偶联:

Unconjugated

产品浓度:

1 mg/ml (Please refer to the vial label for the specific concentration.)

保存温度:

This whole cell lysate is stable for three months at -20ºC

运输温度:

Dry ice

产品形式:

Liquid

存储溶液:

62.5 mM Tris HCl, 2% SDS, 0.005% bromophenol blue, 10% Glycerol, no preservatives.