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折扣/货期| 货号：96-519 | 规格：0.02 mg | 目录价：￥6405.75 |
产品详情
* 以下信息仅供参考，详情请以原厂网站为准
产品名称:
LIF Recombinant Protein (HEK293 Expressed)
别名:
LIF, CDF, DIA, HILDA, MLPLI
实验应用:
WB
表达系统:
HEK293 cells
保存温度:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
运输温度:
Blue Ice
产品形式:
Lyophilized
存储溶液:
PBS, pH7.4
产地:
美国
生产商:
Prosci
功能与背景:
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo.
Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
纯度:
>95% as determined by SDS-PAGE.
生物活性:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0. 1 ng/mL, corresponding to a specific activity of >1X107 Unit/mg.
Accession #:
AAH69540.1
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